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Invadopodia are key structures involved in tumor metastasis. However, it is unclear whether ER\u03b2 is involved in the promotion of NSCLC metastasis through invadopodia. In our study, we used scanning electron microscopy to observe the formation of invadopodia following the overexpression of ER\u03b2 and treatment with E2. In vitro experiments using multiple NSCLC cell lines demonstrated that ER\u03b2 can increase the formation of invadopodia and cell invasion. Mechanistic studies revealed that ER\u03b2 can upregulate the expression of ICAM1 by directly binding to estrogen\u2010responsive elements (EREs) located on the ICAM1 promoter, which in turn can enhance the phosphorylation of Src\/cortactin. We also confirmed these findings in vivo using an orthotopic lung transplantation mouse model, which validated the results obtained from the in vitro experiments. Finally, we examined the expressions of ER\u03b2 and ICAM1 using immunohistochemistry in both NSCLC tissue and paired metastatic lymph nodes. The results confirmed that ER\u03b2 promotes the formation of invadopodia in NSCLC cells through the ICAM1\/p\u2010Src\/p\u2010Cortactin signaling pathway.<\/jats:p>","DOI":"10.1002\/ijc.34563","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T08:59:26Z","timestamp":1684745966000},"page":"1287-1299","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<scp>ER\u03b2<\/scp>\n                    promoted invadopodia formation\u2010mediated non\u2010small cell lung cancer metastasis via the\n                    <scp>ICAM1<\/scp>\n                    \/\n                    <scp>p\u2010Src<\/scp>\n                    \/\n                    <scp>p\u2010Cortactin<\/scp>\n                    signaling pathway"],"prefix":"10.1002","volume":"153","author":[{"given":"Yangwei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery, Union Hospital, Tongji 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