{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:45:42Z","timestamp":1792071942378,"version":"4.4.0"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,8,6]],"date-time":"2023-08-06T00:00:00Z","timestamp":1691280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The short\u2010tandem\u2010repeats (STR) profiles of MGc80\u20103 and HeLa partially overlap, raising suspicion of contamination in the MGc80\u20103 cell line. However, there has not been any relevant study demonstrating whether MGc80\u20103 was fully replaced by HeLa cells, just mixed with HeLa cells (co\u2010existing), or was a somatic hybrid with HeLa cells. In addition to STR profiling, various approaches, including single nucleotide polymorphisms genotyping, polymerase chain reaction, screening for human papillomaviruses type 18 (HPV\u201018) fragment, chromosome karyotyping, pathological examination of xenografts, tissue\u2010specific\u201090\u2010gene expression signature and high\u2010throughput RNA sequencing were used to determine the nature of MGc80\u20103. Our study found that the abnormal STR profile, partially overlapping with that of HeLa cells (64.62% to 71.64%), could not verify MGc80\u20103 as a HeLa cell line. However, the STR 13.3 repeat allele in the D13S317 locus that seemed to be unique to HeLa cells was detected in MGc80\u20103. Almost all the MGc80\u20103 cells exhibited HPV\u201018 fragments in the genome as well as certain HeLa marker chromosomes, such as M7 and M12. The molecular assay of the 90\u2010gene expression signature still considered MGc80\u20103 as a stomach cancer using an algorithmic analysis. The expression pattern of multiple genes in MGc80\u20103 was quite different from that in HeLa cells, which showed that certain characteristics belonged to gastric cancer cell lines. High throughput RNA sequencing showed the distinct patterns of gene expression in MGc80\u20103. In conclusion, MGc80\u20103 cell line is a somatic hybrid with HeLa cells rather than a pure gastric cancer cell line.<\/jats:p>","DOI":"10.1002\/ijc.34677","type":"journal-article","created":{"date-parts":[[2023,8,6]],"date-time":"2023-08-06T18:27:12Z","timestamp":1691346432000},"page":"155-168","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Multiple approaches revealed <scp>MGc80<\/scp>\u20103 as a somatic hybrid with <scp>HeLa<\/scp> cells rather than a gastric cancer cell line"],"prefix":"10.1002","volume":"154","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2319-3293","authenticated-orcid":false,"given":"Fang","family":"Cao","sequence":"first","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Pathology Peking University Cancer Hospital &amp;Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &amp; School of Basic Medicine Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenli","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &amp; School of Basic Medicine Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7634-282X","authenticated-orcid":false,"given":"Yanhua","family":"Bai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Pathology Peking University Cancer Hospital &amp;Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Pathology Peking University Cancer Hospital &amp;Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &amp; School of Basic Medicine Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaocui","family":"Bian","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &amp; School of Basic Medicine Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3720-4982","authenticated-orcid":false,"given":"Yuqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences &amp; School of Basic Medicine Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,6]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09660-w"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170384"},{"key":"e_1_2_12_4_1","first-page":"257","article-title":"A in vitro cell (MGc80\u20103) of a poorly differentiated mucoid adenocarcinoma of human stomach","volume":"16","author":"Wang K","year":"1983","journal-title":"Acta Biol Exp Sin"},{"key":"e_1_2_12_5_1","first-page":"31","article-title":"Induction of apoptosis in human gastric carcinoma cell line MGC\u2010803 by monoclonal antibody PD4","volume":"20","author":"Xiao H","year":"1998","journal-title":"Zhonghua Zhong Liu Za Zhi"},{"key":"e_1_2_12_6_1","first-page":"183","article-title":"Establishment of drug resistant cell line of MGC\u2010803 and analysis of differential secretome","volume":"46","author":"Huang H","year":"2014","journal-title":"Beijing Da Xue Xue Bao Yi Xue Ban"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/CAD.0000000000000601"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2852"},{"key":"e_1_2_12_9_1","first-page":"647","article-title":"The short\u2010tandem\u2010repeat profiling as the gold standard for cell authentication","volume":"42","author":"Liu Y","year":"2013","journal-title":"Chin J Pathol"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yamp.2018.07.005"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.80669"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.184.4141.1093"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/259211a0"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-022-02417-4"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171435"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.3507"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbaa093"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-022-03318-6"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.722808"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2020.06.005"},{"key":"e_1_2_12_21_1","first-page":"288","article-title":"The invasive behavior of two human nasopharyngeal carcinoma (NPC) cell lines CNE\u20101, CNE\u20102Z in vitro","volume":"20","author":"Kong FY","year":"1991","journal-title":"Zhonghua Bing Li Xue Za Zhi"},{"key":"e_1_2_12_22_1","first-page":"153","article-title":"Relationship between invasion and metastasis of tumor cells","volume":"6","author":"Gao J","year":"1991","journal-title":"Chin Med Sci J"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23374"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01457-14"},{"key":"e_1_2_12_25_1","first-page":"329","article-title":"Cell line individualization by STR multiplex system in the cell bank found cross\u2010contamination between ECV304 and EJ\u20101\/T24","volume":"18","author":"Tanabe H","year":"1999","journal-title":"Tissue Cult Res Commun"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32639"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14397"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2023.102853"},{"key":"e_1_2_12_29_1","first-page":"337","article-title":"Genotyping and linkage disequilibrium analysis of 67 SNP loci on X chromosome","volume":"27","author":"Li L","year":"2011","journal-title":"J Forensic Med"},{"key":"e_1_2_12_30_1","first-page":"945","article-title":"George Otto Gey. (1899\u20101970). The HeLa cell and a reappraisal of its origin","volume":"38","author":"Jones HW","year":"1971","journal-title":"Obstet Gynecol"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000132342"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/01443615.2019.1634030"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1159\/000132579"},{"key":"e_1_2_12_34_1","first-page":"141","article-title":"Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping","volume":"59","author":"Macville M","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116218"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.113.005777"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.1965.64.1.31"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200303000-00003"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.4132\/jptm.2020.06.01"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120-021-01163-y"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940040302"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-5492-9"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2015-0081-OA"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2021.101051"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2012.04.001"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-020-1154-2"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12082197"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2014.3152"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02893-6"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.927411"},{"key":"e_1_2_12_51_1","first-page":"319","article-title":"Matrix Gla protein in tumoral pathology","volume":"89","author":"Gheorghe SR","year":"2016","journal-title":"Clujul Med"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23210"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27931"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25242"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.34677","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T06:04:08Z","timestamp":1699855448000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.34677"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,6]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/ijc.34677"],"URL":"https:\/\/doi.org\/10.1002\/ijc.34677","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,6]]},"assertion":[{"value":"2023-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}