{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T17:39:58Z","timestamp":1793813998338,"version":"4.6.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,8,26]],"date-time":"2023-08-26T00:00:00Z","timestamp":1693008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010414","name":"Health Research Board","doi-asserted-by":"publisher","award":["CARG29\/2012"],"award-info":[{"award-number":["CARG29\/2012"]}],"id":[{"id":"10.13039\/100010414","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There are currently several validated HPV tests. However, longitudinal data which spans appropriate age ranges, as well as evaluation of potential screening algorithms are necessary for screening programmes choice of test. The objective of our study was to evaluate the performance of HPV mRNA and HPV DNA testing, including partial genotyping, in routine cervical screening. As part of the CERVIVA HPV Primary Screening Study, ThinPrep samples from 10\u2009150 women were tested for HPV mRNA using the Aptima HPV assay and HPV DNA using the Cobas 4800 HPV test. HPV mRNA\u2010positive women were further assessed with the Aptima genotyping assay for HPV 16\/18\/45. Baseline cytology and prospective follow\u2010up data were collected. The performance of the two tests was examined over 42\u2009months (to date). HPV mRNA demonstrated equivalent sensitivity to HPV DNA testing for detection of CIN2+ (93.2% [92.4\u201093.9] vs 92.8% [92.0\u201093.6], respectively) and CIN3+ (94.6% [93.8\u201095.3] vs 94.6% [93.8\u201095.3]). HPV mRNA testing had significantly higher specificity compared to HPV DNA for detection of CIN2+ (84.0% [83.5\u201084.5] vs 80.8% [80.2\u201081.4], respectively) and CIN3+ (88.44% [88.2\u201088.6] vs 85.62 [85.4\u201085.9]). The proportion of CIN2+ and CIN3+, over 3\u2009years (42\u2009months), in HPV\u2010negative women was comparable for both RNA (0.20% and 0.10%) and DNA (0.22% and 0.11%). Genotyping data was comparable across both assay platforms. In the context of HPV primary screening HPV mRNA testing has potential to reduce triage tests and follow\u2010up tests at 12\u2009months compared to DNA testing, with no significant difference in detection of CIN2+ and CIN3+.<\/jats:p>","DOI":"10.1002\/ijc.34685","type":"journal-article","created":{"date-parts":[[2023,8,26]],"date-time":"2023-08-26T11:54:16Z","timestamp":1693050856000},"page":"53-64","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Performance of the <scp>HPV E6<\/scp>\/<scp>E7 mRNA<\/scp> Aptima <scp>HPV<\/scp> assay combined with partial genotyping compared with the <scp>HPV DNA<\/scp> Cobas 4800 <scp>HPV<\/scp> test for use in primary screening: Results from the <scp>CERVIVA HPV<\/scp> primary screening study in Ireland"],"prefix":"10.1002","volume":"154","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4242-3533","authenticated-orcid":false,"given":"Christine","family":"White","sequence":"first","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin Ireland"},{"name":"Discipline of Histopathology St. James' University Hospital, Trinity College Dublin  Dublin Ireland"},{"name":"The Coombe Hospital  Dublin 8 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Reynolds","sequence":"additional","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin Ireland"},{"name":"The Coombe Hospital  Dublin 8 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Murphy","sequence":"additional","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin 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Pathology Laboratory, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imogen","family":"Sharkey Ochoa","sequence":"additional","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grainne","family":"Glesson","sequence":"additional","affiliation":[{"name":"CervicalCheck, National Screening Service, Kings Inns House  Dublin 1 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noirin","family":"Russell","sequence":"additional","affiliation":[{"name":"CervicalCheck, National Screening Service, Kings Inns House  Dublin 1 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Nuttall","sequence":"additional","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prerna","family":"Tewari","sequence":"additional","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin Ireland"},{"name":"Discipline of Histopathology St. James' University Hospital, Trinity College Dublin  Dublin Ireland"},{"name":"Trinity St James Cancer Institute, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Wright","sequence":"additional","affiliation":[{"name":"CervicalCheck, National Screening Service, Kings Inns House  Dublin 1 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"O'Toole","sequence":"additional","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin Ireland"},{"name":"Discipline of Histopathology St. James' University Hospital, Trinity College Dublin  Dublin Ireland"},{"name":"Trinity St James Cancer Institute, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Sharp","sequence":"additional","affiliation":[{"name":"Population Health Sciences Institute Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grainne","family":"Flannelly","sequence":"additional","affiliation":[{"name":"National Maternity Hospital  Dublin 2 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"O'Leary","sequence":"additional","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin Ireland"},{"name":"Discipline of Histopathology St. James' University Hospital, Trinity College Dublin  Dublin Ireland"},{"name":"The Coombe Hospital  Dublin 8 Ireland"},{"name":"Trinity St James Cancer Institute, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cara M.","family":"Martin","sequence":"additional","affiliation":[{"name":"TCD CERVIVA Molecular Pathology Laboratory, Trinity College Dublin  Dublin Ireland"},{"name":"Discipline of Histopathology St. James' University Hospital, Trinity College Dublin  Dublin Ireland"},{"name":"The Coombe Hospital  Dublin 8 Ireland"},{"name":"Trinity St James Cancer Institute, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"CERVIVA the Irish Cervical Screening Research Consortium","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,26]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"WHO Guidelines for Screening and Treatment of Precancerous Lesions for Cervical Cancer Prevention","author":"WHO","year":"2013"},{"key":"e_1_2_12_3_1","volume-title":"Health Technology 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