{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:22:34Z","timestamp":1791300154511,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:00:00Z","timestamp":1695945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011541","name":"Division of Cancer Epidemiology and Genetics, National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011541","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2024,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The World Health Organization recommends human papillomavirus (HPV) testing for cervical screening. Extended genotyping can identify the highest\u2010risk HPV\u2010positive women. An inexpensive, rapid, mobile isothermal amplification assay (ScreenFire HPV RS test) was recently redesigned to yield four channels ordered by cancer risk (ie, hierarchical approach): HPV16, HPV18\/45, HPV31\/33\/35\/52\/58 and HPV39\/51\/56\/59\/68. Stored specimens from 2076 women (mean age 30.9) enrolled in a colposcopy clinic, with high HPV prevalence, were tested with ScreenFire. We calculated hierarchical channel positivity and non\u2010hierarchical channel and type positivity, according to histologic diagnosis (256 cancer, 350 cervical intraepithelial neoplasia [CIN]3, 409 CIN2, 1020\u2009&lt;\u2009CIN2) and known virologic reference results (Linear Array and TypeSeq). Additionally, we analyzed ScreenFire time\u2010to\u2010positive up to 60\u2009min by channel and histology. Overall clinical sensitivity for CIN3+ was 94.7% (95% confidence interval 92.6\u201096.4), similar to Linear Array (92.3, 89.7\u201094.3) and TypeSeq (96.0, 93.9\u201097.6). Sensitivity was high for all types and channels. The hierarchical approach was well in line with HPV typing and histologic diagnosis, prioritizing higher risk women having HPV16 and precancer. For HPV16, time\u2010to\u2010positive was shorter in women with precancer. ScreenFire showed excellent agreement with research reference typing tests and detection of CIN2+. Risk\u2010based type results could help guide clinical management of HPV\u2010positive women. Time\u2010to\u2010positive combined with genotyping might be useful. ScreenFire is rapid, mobile, relatively inexpensive and designed for implementation of HPV\u2010based screening and management, including in lower\u2010resource settings. Further validation in screening by self\u2010sampling and practical effectiveness merit evaluation.<\/jats:p>","DOI":"10.1002\/ijc.34698","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T12:50:31Z","timestamp":1695991831000},"page":"241-250","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["A rapid HPV typing assay to support global cervical cancer screening and risk\u2010based management: A cross\u2010sectional study"],"prefix":"10.1002","volume":"154","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3661-0842","authenticated-orcid":false,"given":"Federica","family":"Inturrisi","sequence":"first","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute, National Institutes of Health  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"de Sanjos\u00e9","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute, National Institutes of Health  Rockville Maryland USA"},{"name":"ISGlobal  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanan T.","family":"Desai","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute, National Institutes of Health  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casey","family":"Dagnall","sequence":"additional","affiliation":[{"name":"Cancer Genomics Research Laboratory, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc.  Frederick Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didem","family":"Egemen","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute, National Institutes of Health  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Befano","sequence":"additional","affiliation":[{"name":"Information Management Services Inc.  Calverton Maryland USA"},{"name":"Department of Epidemiology University of Washington School of Public Health  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cecilia","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute, National Institutes of Health  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose A.","family":"Jeronimo","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute, National Institutes of Health  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary E.","family":"Zuna","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Oklahoma Health Sciences Center  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Cancer Genomics Research Laboratory, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc.  Frederick Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sepideh","family":"Farhat Nozzari","sequence":"additional","affiliation":[{"name":"Department of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan L.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Oklahoma Health Sciences Center  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca B.","family":"Perkins","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Boston Medical Center\/Boston University School of Medicine  Boston Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1251-0836","authenticated-orcid":false,"given":"Nicolas","family":"Wentzensen","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute, National Institutes of Health  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel M.","family":"Palefsky","sequence":"additional","affiliation":[{"name":"Department of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Schiffman","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute, National Institutes of Health  Rockville Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,29]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"WHO comprehensive cervical cancer control: a guide to essential practice","author":"World Health Organization","year":"2014"},{"key":"e_1_2_12_3_1","volume-title":"WHO Guideline for Screening and Treatment of Cervical Pre\u2010Cancer Lesions for Cervical Cancer Prevention","author":"World Health Organization","year":"2021"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(22)00241-8"},{"key":"e_1_2_12_5_1","volume-title":"IARC Monographs on the Evaluation of Carcinogenic Risks to Humans","author":"World Health Organization","year":"2007"},{"key":"e_1_2_12_6_1","volume-title":"IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 100B","author":"World Health Organization","year":"2012"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2015.11.015"},{"key":"e_1_2_12_8_1","unstructured":"Swedish National Board of Health and Welfare's: Cervical Cancer Prevention.Recommendations regarding screening. 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