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However, marketed anti\u2010CD25 antibodies (Basiliximab and Daclizumab) were originally developed as immunosuppressive drugs to prevent graft rejection, because these antibodies can block IL\u20102 binding to CD25 on Teffs, which in turn destroys the function of Teffs. Recent studies have shown that non\u2010IL\u20102\u2010blocking anti\u2010CD25 antibodies have displayed exciting antitumor effects. Here, we screened out a non\u2010IL\u20102\u2010blocking anti\u2010CD25 monoclonal antibody (mAb) 7B7 by hybridoma technology, and confirmed its antitumor activity via depleting Tregs in a CD25 humanized mouse model. Subsequently, we verified that the humanized 7B7, named as h7B7\u201015S, has comparable activities to 7B7, and that its Treg depletion is further increased when combined with anti\u2010CTLA\u20104, leading to enhanced remodeling of the tumor immune microenvironment. Moreover, our findings reveal that the Fab form of h7B7\u201015S has the ability to deplete Tregs, independent of the Fc region. Taken together, our studies expand the application of anti\u2010CD25 in tumor immunotherapy and provide insight into the underlying mechanism.<\/jats:p>","DOI":"10.1002\/ijc.34823","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T12:38:55Z","timestamp":1704458335000},"page":"1285-1297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>Non\u2010IL<\/scp>\u20102\u2010blocking <scp>anti\u2010CD25<\/scp> antibody inhibits tumor growth by depleting Tregs and has synergistic effects with <scp>anti\u2010CTLA<\/scp>\u20104 therapy"],"prefix":"10.1002","volume":"154","author":[{"given":"Yujia","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyin","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinhuai","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuiyu","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangbing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Head and Neck Oncology, West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy\/Collaborative Innovation Center for Biotherapy, West China Hospital Sichuan University  Chengdu China"},{"name":"Precision Medicine Key Laboratory of Sichuan Province &amp; 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