{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T02:05:02Z","timestamp":1789524302640,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171991"],"award-info":[{"award-number":["82171991"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2023J011125"],"award-info":[{"award-number":["2023J011125"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2020J01154"],"award-info":[{"award-number":["2020J01154"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2020J01839"],"award-info":[{"award-number":["2020J01839"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2022J01170"],"award-info":[{"award-number":["2022J01170"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2020J011008"],"award-info":[{"award-number":["2020J011008"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2024,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ductal carcinoma in situ with microinvasion (DCISM) is a challenging subtype of breast cancer with controversial invasiveness and prognosis. Accurate diagnosis of DCISM from ductal carcinoma in situ (DCIS) is crucial for optimal treatment and improved clinical outcomes. However, there are often some suspicious small cancer nests in DCIS, and it is difficult to diagnose the presence of intact myoepithelium by conventional hematoxylin and eosin (H&amp;E) stained images. Although a variety of biomarkers are available for immunohistochemical (IHC) staining of myoepithelial cells, no single biomarker is consistently sensitive to all tumor lesions. Here, we introduced a new diagnostic method that provides rapid and accurate diagnosis of DCISM using multiphoton microscopy (MPM). Suspicious foci in H&amp;E\u2010stained images were labeled as regions of interest (ROIs), and the nuclei within these ROIs were segmented using a deep learning model. MPM was used to capture images of the ROIs in H&amp;E\u2010stained sections. The intensity of two\u2010photon excitation fluorescence (TPEF) in the myoepithelium was significantly different from that in tumor parenchyma and tumor stroma. Through the use of MPM, the myoepithelium and basement membrane can be easily observed via TPEF and second\u2010harmonic generation (SHG), respectively. By fusing the nuclei in H&amp;E\u2010stained images with MPM images, DCISM can be differentiated from suspicious small cancer clusters in DCIS. The proposed method demonstrated good consistency with the cytokeratin 5\/6 (CK5\/6) myoepithelial staining method (kappa coefficient\u2009=\u20090.818).<\/jats:p>","DOI":"10.1002\/ijc.34855","type":"journal-article","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T08:00:45Z","timestamp":1706169645000},"page":"1802-1813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Improving the diagnosis of ductal carcinoma in situ with microinvasion without immunohistochemistry: An innovative method with H&amp;E\u2010stained and multiphoton microscopy images"],"prefix":"10.1002","volume":"154","author":[{"given":"Xiahui","family":"Han","sequence":"first","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulan","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shichao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianhuang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lida","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Physics and Electronic Information Engineering Minjiang University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"},{"name":"College of Life Science Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenlin","family":"Zhan","sequence":"additional","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Mechanical Engineering and Automation Fuzhou University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianli","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyong","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Pathology Fujian Medical University Union Hospital  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8519-4462","authenticated-orcid":false,"given":"Jianxin","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djp482"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2013.07.005"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-019-0478-6"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jbi\/wbz039"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2014.54"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(03)00166-1"},{"key":"e_1_2_11_9_1","first-page":"139p","volume-title":"AJCC Cancer Staging Manual","author":"Amin MB","year":"2017"},{"key":"e_1_2_11_10_1","first-page":"267","volume-title":"Biopsy Interpretation of the Breast","author":"Schnitt SJ","year":"2012"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2015.11.003"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbj.13897"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200301000-00009"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318180431d"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-016-1909-x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt899"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri935"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth818"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0832308100"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.55921"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2021.06.011"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-021-02146-7"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-5497-4"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1909243116"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aau5603"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2019.12.030"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1109\/RBME.2013.2295804"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11831-019-09366-4"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.media.2019.101563"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1201\/9780429258589"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/1536867X0400400404"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2006.01.001"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.4048\/jbc.2018.21.2.197"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-019-07556-9"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-017-4572-2"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2020.04.031"},{"key":"e_1_2_11_37_1","first-page":"20p","volume-title":"An Introduction to Pathology","author":"Kierszenbaum A","year":"2019"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-07244-2"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.4472"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038655"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.OE.61.7.073103"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202000141"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-019-1936-x"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2013.07.010"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-022-06553-z"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-4-38"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.34855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T09:10:06Z","timestamp":1710321006000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.34855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,25]]},"references-count":45,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,5,15]]}},"alternative-id":["10.1002\/ijc.34855"],"URL":"https:\/\/doi.org\/10.1002\/ijc.34855","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,25]]},"assertion":[{"value":"2023-09-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}