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By using microfluidic or density gradient CTC enrichment in combination with immunofluorescent (IF) staining or qPCR of CTC\u2010related transcripts, we achieved a 60.8% to 88.0% positivity in SCLC blood samples. Epithelial and neuroendocrine transcripts including the druggable target <jats:italic>DLL3<\/jats:italic> were associated with shorter overall survival (OS), indicating the clinical value of these markers in terms of differential diagnosis and treatment decisions. High CTC counts and the presence of CTC duplets detected by IF staining were prognostic for OS, and thus may serve as indicators of disease progression or therapy failure. In patient samples with high CTC load detected by IF staining, a concordance of the transcripts positivity in circulating free plasma RNA and CTCs was observed. Our data emphasize the role of CTCs and CTC\u2010related transcripts and underline the clinical value of liquid biopsy analysis in SCLC.<\/jats:p>","DOI":"10.1002\/ijc.34886","type":"journal-article","created":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T13:43:40Z","timestamp":1707918220000},"page":"2189-2199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prognostic significance of circulating tumor cells and tumor related transcripts in small cell lung cancer: A step further to clinical implementation"],"prefix":"10.1002","volume":"154","author":[{"given":"Eva","family":"Welsch","sequence":"first","affiliation":[{"name":"Molecular Oncology Group, Department of Obstetrics and Gynecology, Comprehensive Cancer Center Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Holzer","sequence":"additional","affiliation":[{"name":"Molecular 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