{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:43:21Z","timestamp":1786977801520,"version":"build-2736575974"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:00:00Z","timestamp":1715558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["742432"],"award-info":[{"award-number":["742432"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Endometrial cancer (EC) is the most prevalent gynaecological cancer in high\u2010income countries and its incidence is continuing to rise sharply. Simple and objective tools to reliably detect women with EC are urgently needed. We recently developed and validated the DNA methylation (DNAme)\u2010based women's cancer risk identification\u2014quantitative polymerase chain reaction test for endometrial cancer (WID\u2010qEC) test that could address this need. Here, we demonstrate that the stability of the WID\u2010qEC test remains consistent regardless of: (i) the cervicovaginal collection device and sample media used (Cervex brush and PreservCyt or FLOQSwab and eNAT), (ii) the collector of the specimen (gynaecologist\u2010 or patient\u2010based), and (iii) the precise sampling site (cervical, cervicovaginal and vaginal). Furthermore, we demonstrate sample stability in eNAT medium for 7\u2009days at room temperature, greatly facilitating the implementation of the test into diagnostic laboratory workflows. When applying FLOQSwabs (Copan) in combination with the eNAT (Copan) sample collection media, the sensitivity and specificity of the WID\u2010qEC test to detect uterine (i.e., endometrial and cervical) cancers in gynaecologist\u2010taken samples was 92.9% (95% confidence interval [CI] =\u200975.0%\u201398.8%) and 98.6% (95% CI\u2009=\u200991.7%\u201399.9%), respectively, whilst the sensitivity and specificity in patient collected self\u2010samples was 75.0% (95% CI\u2009=\u200947.4%\u201391.7%) and 100.0% (95% CI\u2009=\u200993.9%\u2013100.0%), respectively. Taken together these data confirm the robustness and clinical potential of the WID\u2010qEC test.<\/jats:p>","DOI":"10.1002\/ijc.35000","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T09:53:40Z","timestamp":1715594020000},"page":"800-806","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["High performance of the\n                    <scp>DNA<\/scp>\n                    methylation\u2010based\n                    <scp>WID\u2010qEC<\/scp>\n                    test for detecting uterine cancers independent of sampling modalities"],"prefix":"10.1002","volume":"155","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7322-2427","authenticated-orcid":false,"given":"Ojone","family":"Illah","sequence":"first","affiliation":[{"name":"Department of Women's Cancer UCL EGA Institute for Women's Health, University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1675-4654","authenticated-orcid":false,"given":"Malcolm","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Women's Cancer UCL EGA Institute for Women's Health, University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6540-8443","authenticated-orcid":false,"given":"Elisa","family":"Redl","sequence":"additional","affiliation":[{"name":"European Translational Oncology Prevention and Screening (EUTOPS) Institute, Universit\u00e4t Innsbruck  Hall in Tirol Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1274-327X","authenticated-orcid":false,"given":"James E.","family":"Barrett","sequence":"additional","affiliation":[{"name":"European Translational Oncology Prevention and Screening (EUTOPS) Institute, Universit\u00e4t Innsbruck  Hall in Tirol Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3065-9888","authenticated-orcid":false,"given":"Lena","family":"Schreiberhuber","sequence":"additional","affiliation":[{"name":"European Translational Oncology Prevention and Screening (EUTOPS) Institute, Universit\u00e4t Innsbruck  Hall in Tirol Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1572-498X","authenticated-orcid":false,"given":"Chiara","family":"Herzog","sequence":"additional","affiliation":[{"name":"European Translational Oncology Prevention and Screening (EUTOPS) Institute, Universit\u00e4t Innsbruck  Hall in Tirol Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5027-2309","authenticated-orcid":false,"given":"Charlotte D.","family":"Vavourakis","sequence":"additional","affiliation":[{"name":"European Translational Oncology Prevention and Screening (EUTOPS) Institute, Universit\u00e4t Innsbruck  Hall in Tirol Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Women's Cancer UCL EGA Institute for Women's Health, University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iona","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Women's Cancer UCL EGA Institute for Women's Health, University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Reisel","sequence":"additional","affiliation":[{"name":"Department of Women's Cancer UCL EGA Institute for Women's Health, University College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhivya","family":"Chandrasekaran","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology University College London Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kostas","family":"Doufekas","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology University College London Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5041-1145","authenticated-orcid":false,"given":"Radha","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology University College London Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis C.","family":"Kotsopoulos","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology University College London Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"MacDonald","sequence":"additional","affiliation":[{"name":"Department of Gynaecological Oncology University College London Hospital  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