{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:39:46Z","timestamp":1773419986092,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nasopharyngeal carcinoma (NPC) risk prediction models based on Epstein\u2013Barr virus (EBV)\u2010antibody testing have shown potential for screening of NPC; however, the long\u2010term stability is unclear. Here, we investigated the kinetics of two EBV\u2010antibody NPC risk scores within the Taiwan NPC Multiplex Family Study. Among 545 participants with multiple blood samples, we evaluated the stability of a 2\u2010marker enzyme\u2010linked immunosorbent assay score and 13\u2010marker multiplex serology score using the intra\u2010class correlation coefficient (ICC) by fitting a linear mixed model that accounted for the clustering effect of multiple measurements per subject and age. We also estimated the clustering of positive tests using Fleiss's kappa statistic. Over an average 20\u2010year follow\u2010up, the 2\u2010marker score showed high stability over time, whereas the 13\u2010marker score was more variable (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.05). Case\u2013control status is associated with the kinetics of the antibody response, with higher ICCs among cases. Positive tests were more likely to cluster within the same individual for the 2\u2010marker score than the 13\u2010marker score (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.05). The 2\u2010marker score had an increase in specificity from ~90% for single measurement to ~96% with repeat testing. The 13\u2010marker score had a specificity of ~73% for a single measurement that increased to ~92% with repeat testing. Among individuals who developed NPC, none experienced score reversion. Our findings suggest that repeated testing could improve the specificity of NPC screening in high\u2010risk NPC multiplex families. Further studies are required to determine the impact on sensitivity, establish optimal screening intervals, and generalize these findings to general population settings in high\u2010risk regions.\n                  <\/jats:p>","DOI":"10.1002\/ijc.35037","type":"journal-article","created":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T06:52:58Z","timestamp":1717224778000},"page":"1400-1408","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Kinetics of EBV antibody\u2010based NPC risk scores in Taiwan NPC multiplex families"],"prefix":"10.1002","volume":"155","author":[{"given":"Wan\u2010Lun","family":"Hsu","sequence":"first","affiliation":[{"name":"Data Science Center, College of Medicine Fu\u2010Jen Catholic University  New Taipei City Taiwan"},{"name":"College of Medicine Fu\u2010Jen Catholic University  New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Tao","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute  Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Fu","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute  Bethesda Maryland USA"},{"name":"School of Statistics and Data Science Nankai University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly J.","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute  Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Simon","sequence":"additional","affiliation":[{"name":"Division of Infections and Cancer Epidemiology German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ)  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tseng\u2010Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National Taiwan University Hospital and National Taiwan University, College of Medicine  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6996-9069","authenticated-orcid":false,"given":"Chien\u2010Jen","family":"Chen","sequence":"additional","affiliation":[{"name":"Genomics Research Center Academia Sinica  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7538-3582","authenticated-orcid":false,"given":"Alisa M.","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute  Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3832-6586","authenticated-orcid":false,"given":"Kai","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute  Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0257-2363","authenticated-orcid":false,"given":"Allan","family":"Hildesheim","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute  Bethesda Maryland USA"},{"name":"Agencia Costarriciense de Investigaciones Biologicas  San Jose Costa Rica"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Waterboer","sequence":"additional","affiliation":[{"name":"Division of Infections and Cancer Epidemiology German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ)  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National Taiwan University Hospital and National Taiwan University, College of Medicine  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0136-651X","authenticated-orcid":false,"given":"Zhiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics National Cancer Institute  Bethesda Maryland 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