{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T21:09:39Z","timestamp":1792271379643,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008903","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["MOHW109\u2010TDU\u2010B\u2010212\u2010114005"],"award-info":[{"award-number":["MOHW109\u2010TDU\u2010B\u2010212\u2010114005"]}],"id":[{"id":"10.13039\/100008903","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008903","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["MOHW110\u2010TDU\u2010B\u2010212\u2010114005"],"award-info":[{"award-number":["MOHW110\u2010TDU\u2010B\u2010212\u2010114005"]}],"id":[{"id":"10.13039\/100008903","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008903","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["MOHW111\u2010TDU\u2010B\u2010212\u2010114005"],"award-info":[{"award-number":["MOHW111\u2010TDU\u2010B\u2010212\u2010114005"]}],"id":[{"id":"10.13039\/100008903","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004606","name":"Chang Gung Medical Foundation","doi-asserted-by":"publisher","award":["CMRPG3L0591"],"award-info":[{"award-number":["CMRPG3L0591"]}],"id":[{"id":"10.13039\/501100004606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004606","name":"Chang Gung Medical Foundation","doi-asserted-by":"publisher","award":["CMRPG3L0701\/2"],"award-info":[{"award-number":["CMRPG3L0701\/2"]}],"id":[{"id":"10.13039\/501100004606","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We aimed to investigate human papillomavirus (HPV) prevalence and genotype distribution and prognostic factors in vaginal cancer (VC). VC patients who received treatment between 1989 and 2020 were retrospectively reviewed. L1 general polymerase chain reaction (PCR) followed by HPV Blot (King Car, I\u2010Lan, Taiwan) and E6 type\u2010specific\u2010PCR were performed for genotyping firstly. P16 and p53 immunohistochemistry staining was performed. Univariate and multivariate analyses identified predictors of clinical outcomes.79 VC patients were eligible for analysis. 73 patients (92.4%) were squamous cell carcinoma (SCC) and 6 (7.6%) as non\u2010SCC. The median follow\u2010up time was 134.3\u2009months (range 0.9\u2013273.4). Among nine initially HPV\u2010negative cases, seven were identified as being positive through HPV16\/18\/45\/52\/58 whole\u2010genome amplification followed by Sanger sequencing (WGASS). HPV DNA sequences were detected in 98.6% of SCC and 83.3% of non\u2010SCC, respectively, with HPV16 (49.4%), HPV52 (15.2%) and HPV58 (8.9%) being predominant. Patients with paraaortic lymph node (LN) metastasis had a 5\u2010year cancer\u2010specific survival (CSS) rate of 0%. Multivariate analysis revealed that only p16 and stage were significantly correlated with prognosis. Variables with strong correlations (p16\u2010 and HPV\u2010positivity, LN metastasis and stage), were included in models 2\u20135 alternatively. Stage III\/IV (hazard ratio [HR]\u2009=\u20093.64\u20134.56) and LN metastasis (HR\u2009=\u20092.81\u20133.44) were significant negative predictors of CSS, whereas p16\u2010positivity (HR\u2009=\u20090.29\u20130.32) and HPV\u2010positivity (HR\u2009=\u20090.14) were related to better prognosis. In conclusion, 97.5% of VCs were HPV\u2010positive with WGASS. Stage III\/IV and LN metastasis were significant negative predictors, whereas p16\u2010 and HPV\u2010positivity were significantly associated with better prognosis.<\/jats:p>","DOI":"10.1002\/ijc.35105","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T15:27:19Z","timestamp":1721834839000},"page":"1996-2008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Human papillomavirus prevalence, genotype distribution, and prognostic factors of vaginal cancer"],"prefix":"10.1002","volume":"155","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6160-6124","authenticated-orcid":false,"given":"Hsiu\u2010Jung","family":"Tung","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You\u2010Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Keelung Branch and Chang Gung University College of Medicine  Keelung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1256-7018","authenticated-orcid":false,"given":"Chiao\u2010Yun","family":"Lin","sequence":"additional","affiliation":[{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Jie","family":"Liao","sequence":"additional","affiliation":[{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Keelung Branch and Chang Gung University College of Medicine  Keelung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Bin","family":"Pan","sequence":"additional","affiliation":[{"name":"Clinical Trial Center, Linkou Chang Gung Memorial Hospital  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih\u2010Ming","family":"Jung","sequence":"additional","affiliation":[{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Department of Pathology, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Chieh","family":"Wang","sequence":"additional","affiliation":[{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Department of Radiation Oncology, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huei\u2010Jean","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3834-2754","authenticated-orcid":false,"given":"Angel","family":"Chao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung\u2010Hsueh","family":"Chou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1870-7453","authenticated-orcid":false,"given":"Ting\u2010Chang","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6305-540X","authenticated-orcid":false,"given":"Lan\u2010Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Clinical Trial, Department of Medical Research Taichung Veterans General Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9977-9645","authenticated-orcid":false,"given":"Chyong\u2010Huey","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"},{"name":"Gynecologic Cancer Research Center, Chang Gung Memorial Hospital Linkou Branch and Chang Gung University College of Medicine  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpdhp.2016.11.006"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00261-021-03228-z"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.12610"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.2001.6502"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000000171"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/aogs.14260"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2011.04.004"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.13867"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2023.109662"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1438.2006.00520.x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24116"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2008.12.015"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0528-x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0527-y"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.11.2995"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22538"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27354"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33251"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2020.08.036"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2016.03.011"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-019-0437-z"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2011.12.449"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2008.91"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2013.02.004"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2018.10.015"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2014.32"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.12749"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.21037\/tcr-20-1825"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2016.03.009"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.35105","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T11:39:34Z","timestamp":1727955574000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.35105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,24]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/ijc.35105"],"URL":"https:\/\/doi.org\/10.1002\/ijc.35105","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,24]]},"assertion":[{"value":"2024-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}