{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:47:15Z","timestamp":1792201635281,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA 014089"],"award-info":[{"award-number":["P30CA 014089"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019757","name":"Gloria Borges WunderGlo Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019757","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006508","name":"Dhont Family Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006508","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2025,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Myeloid cell leukemia 1 (MCL\u20101) is a member of the B\u2010cell lymphoma 2 protein family and has anti\u2010apoptotic functions. Deregulation of MCL\u20101 has been reported in several cancers, including lung and breast cancer. In the present study, the association of <jats:italic>MCL\u20101<\/jats:italic> expression with molecular features in colorectal cancer (CRC) has been highlighted. CRC samples from Caris Life Sciences (Phoenix, AZ) were analyzed using NextGen DNA sequencing, whole transcriptome sequencing, whole exome sequencing, and immunohistochemistry (IHC); and stratified based on MCL\u20101 expression as top quartile <jats:italic>MCL\u20101<\/jats:italic><jats:sup>high<\/jats:sup> (Q4) and bottom quartile <jats:italic>MCL\u20101<\/jats:italic><jats:sup>low<\/jats:sup> (Q1). Immune cell infiltration (CI) in the tumor microenvironment (TME) was measured using RNA deconvolution analysis (QuanTIseq). <jats:italic>MCL\u20101<\/jats:italic><jats:sup>high<\/jats:sup> tumors were associated with an increased rate of programmed death ligand 1 IHC, higher T cell\u2010inflamed signature, interferon score, microsatellite instability\u2010high and tumor mutational burden\u2010high status. <jats:italic>MCL\u20101<\/jats:italic><jats:sup>high<\/jats:sup> was associated with higher mutation rates of <jats:italic>BCOR<\/jats:italic>, <jats:italic>TP53<\/jats:italic>, <jats:italic>KMT2D<\/jats:italic>, <jats:italic>ASXL1<\/jats:italic>, <jats:italic>KDM6A<\/jats:italic>, <jats:italic>ATM<\/jats:italic>, <jats:italic>MSH6<\/jats:italic>, <jats:italic>SPEN<\/jats:italic>, <jats:italic>KRAS<\/jats:italic>, <jats:italic>STK11<\/jats:italic>, <jats:italic>GNAS<\/jats:italic>, <jats:italic>RNF43<\/jats:italic>, and lower mutation rates of <jats:italic>CDKN1B<\/jats:italic>, <jats:italic>NRAS<\/jats:italic>, and <jats:italic>APC<\/jats:italic>, and copy number amplifications in several genes. <jats:italic>MCL\u20101<\/jats:italic><jats:sup>high<\/jats:sup> TME had higher CI of M1 and M2 macrophages, B cells, natural killer cells, neutrophils, and T\u2010regulatory cells infiltration, and lower CI of myeloid dendritic cells. Higher <jats:italic>MCL\u20101<\/jats:italic> expression is significantly associated with favorable clinical outcomes in CRC cohorts. Our data showed a strong correlation between <jats:italic>MCL\u20101<\/jats:italic> and distinct immune biomarkers and TME CI in CRC. Our findings suggest MCL\u20101 is a potential modulator of antitumor immunity, TME, and biomarker in CRC.<\/jats:p>","DOI":"10.1002\/ijc.35304","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T11:48:53Z","timestamp":1735559333000},"page":"1583-1593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comprehensive characterization of <i>MCL\u20101<\/i> in patients with colorectal cancer: Expression, molecular profiles, and outcomes"],"prefix":"10.1002","volume":"156","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2156-1419","authenticated-orcid":false,"given":"Pooja","family":"Mittal","sequence":"first","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"},{"name":"Department of Zoology, Deshbandhu College University of Delhi  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Battaglin","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmine","family":"Baca","sequence":"additional","affiliation":[{"name":"Caris Life Sciences  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Xiu","sequence":"additional","affiliation":[{"name":"Caris Life Sciences  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Farrell","sequence":"additional","affiliation":[{"name":"Caris Life Sciences  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Soni","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Ho","family":"Lo","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesly","family":"Torres\u2010Gonzalez","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Algaze","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya","family":"Jayachandran","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karam","family":"Ashouri","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Wong","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Yu","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0018-3903","authenticated-orcid":false,"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin A.","family":"Weinberg","sequence":"additional","affiliation":[{"name":"Ruesch Center for The Cure of Gastrointestinal Cancers, Lombardi Comprehensive Cancer Center Georgetown University Medical Center  Washington DC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emil","family":"Lou","sequence":"additional","affiliation":[{"name":"Division of Hematology, Oncology and Transplantation University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony F.","family":"Shields","sequence":"additional","affiliation":[{"name":"Department of Oncology, Karmanos Cancer Institute Wayne State University  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard M.","family":"Goldberg","sequence":"additional","affiliation":[{"name":"West Virginia University Cancer Institute  Morgantown West Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John L.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Ruesch Center for The Cure of Gastrointestinal Cancers, Lombardi Comprehensive Cancer Center Georgetown University Medical Center  Washington DC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Goel","sequence":"additional","affiliation":[{"name":"Robert Wood Johnson Medical School Rutgers Cancer Institute of New Jersey  New Brunswick New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3211-8471","authenticated-orcid":false,"given":"Indrakant K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"},{"name":"Department of Zoology, Deshbandhu College University of Delhi  New Delhi India"},{"name":"Delhi School of Public Health, Institute of Eminence University of Delhi  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2178-9568","authenticated-orcid":false,"given":"Heinz\u2010Josef","family":"Lenz","sequence":"additional","affiliation":[{"name":"Norris Comprehensive Cancer Center, Keck School of Medicine University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9123889"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-0116-z"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21763"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.07.166"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010020\u201001601\u20109"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.03.017"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04334-y"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-3242"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.45363"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2022.100798"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073\u2010019\u20100638\u20106"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1609376113"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1372"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.5306\/wjco.v12.i11.1000"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.11.105"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10102515"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29750"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920\u2010023\u201001555\u20102"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1090838\/full"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01052\/full"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.343"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/09636897221124485"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2649"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.625783\/full"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010020\u201001302\u20106"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-020-0436-7"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047045"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22147564"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5539"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1100\/2011\/131539"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22083811"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02564-6"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12020332"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.35304","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T07:43:54Z","timestamp":1739519034000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.35304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,30]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,4,15]]}},"alternative-id":["10.1002\/ijc.35304"],"URL":"https:\/\/doi.org\/10.1002\/ijc.35304","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,30]]},"assertion":[{"value":"2024-08-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}