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Current boron carriers like sodium mercaptoundecahydrododecaborate (BSH) and L\u2010<jats:italic>p<\/jats:italic>\u2010boronophenylalanine (BPA) face limitations in specificity and solubility. Our recently developed 6\u2010<jats:italic>O<\/jats:italic>\u2010(<jats:italic>o<\/jats:italic>\u2010carboranylmethyl)\u2010<jats:sc>d<\/jats:sc>\u2010glucopyranose (B\u2010Glc) shows promise as an alternative, demonstrating strong interactions with glucose transporters in human head and neck squamous cell carcinoma (HNSCC) CAL 27 cells in vitro. This study aims to extend in vitro investigations to three additional patient\u2010derived human HNSCC cell lines (UT\u2010SCC\u201014, UT\u2010SCC\u201028, and UT\u2010SCC\u201042B) and to further evaluate in vivo pharmacokinetics in selected HNSCC tumor xenografts. The B\u2010Glc showed superior uptake and favorable kinetic parameters compared to BPA and BSH in all tested cell lines. 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