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Here, we integrated single chain variable fragment (scFv) development with CAR T cell generation based on a newly developed scFv phagemid library. High\u2010throughput long\u2010read PacBio sequencing identified 4.5 \u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    unique full\u2010length scFv proteins within the generated library. As a proof of principle, we screened for scFvs targeting C\u2010type lectin\u2010like molecule\u20101 (CLL1) with subsequent cloning into a third generation retroviral CAR backbone. Functional assays revealed the specificity and potency of these CAR T cells in targeting CLL1\u2010positive AML cells in vitro. In vivo studies reduced tumor burden and improved survival rates compared to controls. Taken together, screening for tumor\u2010specific scFvs against CLL1 can rapidly generate AML\u2010specific CAR T cells with effective tumor killing in vivo.\n                  <\/jats:p>","DOI":"10.1002\/ijc.70146","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T19:30:11Z","timestamp":1759433411000},"page":"994-1007","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrated\n                    <scp>scFv<\/scp>\n                    identification and\n                    <scp>CAR<\/scp>\n                    T cell generation for\n                    <scp>AML<\/scp>\n                    targeting in vivo"],"prefix":"10.1002","volume":"158","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4961-2660","authenticated-orcid":false,"given":"Yi","family":"Liu","sequence":"first","affiliation":[{"name":"Medical Faculty Heidelberg and Department of Internal Medicine V, Hematology, 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