{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:09:43Z","timestamp":1791220183319,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2026,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aimed to assess the diagnostic\u2010support and triage value of PAX1\/JAM3 methylation testing for identifying high\u2010grade cervical lesions among postmenopausal women referred for colposcopy. A total of 216 women aged \u226550\u2009years who underwent colposcopy due to positive high\u2010risk human papillomavirus (hrHPV)\/cytology detection and\/or abnormal clinical symptoms were included, and 212 women aged &lt;50\u2009years matched 1:1 by hrHPV and cytology results as the control group. The effectiveness of\n                    <jats:italic>PAX1<\/jats:italic>\n                    \/\n                    <jats:italic>JAM3<\/jats:italic>\n                    methylation in detecting high\u2010grade cervical intraepithelial neoplasia (CIN) was compared to traditional screening methods.\n                    <jats:italic>PAX1\/JAM3<\/jats:italic>\n                    methylation showed a sensitivity of 93.2%[85.7%\u2013100%] for detecting CIN2+ lesions (97.2%[91.9%\u2013100%] for CIN3+), with a specificity of 93.6%[89.9%\u201397.3%] for CIN1\u2010lesions, outperforming liquid\u2010based cytology (LBC) (CIN2+\/CIN3+ sensitivity: 75%[60.9%\u201389.1%]; specificity: 52.3%[44.9%\u201359.8%]) and the combination of LBC and hrHPV tests according to current guidelines (CIN2+\/CIN3+ sensitivity: 81.8%[70.4%\u201393.2%]\/83.3%[71.2%\u201395.5%]; specificity: 45.3%[37.9%\u201352.8%]). Methylation detection successfully identified two adenocarcinoma cases with negative hrHPV and LBC, as well as 7 patients with non\u201016\/18 hrHPV infection that were missed by LBC. The methylation levels of\n                    <jats:italic>PAX1<\/jats:italic>\n                    and\n                    <jats:italic>JAM3<\/jats:italic>\n                    were elevated in elderly women with CIN2+ compared to younger women, and showed no association with HPV types. In conclusion,\n                    <jats:italic>PAX1\/JAM3<\/jats:italic>\n                    methylation testing showed promising diagnostic\u2010support performance among postmenopausal women referred for colposcopy, suggesting potential utility as a supplementary detection method to improve diagnostic accuracy in older women.\n                  <\/jats:p>","DOI":"10.1002\/ijc.70245","type":"journal-article","created":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T04:42:29Z","timestamp":1763181749000},"page":"1116-1125","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Enhanced diagnostic accuracy of high\u2010grade cervical intraepithelial neoplasia in postmenopausal women through\n                    <scp>\n                      <i>PAX1<\/i>\n                    <\/scp>\n                    \/\n                    <scp>\n                      <i>JAM3<\/i>\n                    <\/scp>\n                    methylation analysis"],"prefix":"10.1002","volume":"158","author":[{"given":"Huanzi","family":"Peng","sequence":"first","affiliation":[{"name":"Medical Laboratory Center The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Medical Laboratory Center The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Medical Laboratory Center QingHai Red Cross Hospital  Qinghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Medical Laboratory Center The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Peng","sequence":"additional","affiliation":[{"name":"Medical Laboratory Center The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Beijing Origin\u2010Poly Bio\u2010Tec Co., Ltd  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Beijing Origin\u2010Poly Bio\u2010Tec Co., Ltd  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"He","sequence":"additional","affiliation":[{"name":"Medical Laboratory Center The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wene","family":"Liu","sequence":"additional","affiliation":[{"name":"Medical Laboratory Center The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Tan","sequence":"additional","affiliation":[{"name":"Medical Laboratory Center The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking Union Medical College Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4413-3983","authenticated-orcid":false,"given":"Xiaobing","family":"Xie","sequence":"additional","affiliation":[{"name":"Medical Laboratory Center The First Hospital of Hunan University of Chinese Medicine  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.12.008"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.currproblcancer.2018.06.003"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clon.2021.07.001"},{"key":"e_1_2_12_7_1","first-page":"221","article-title":"Cancer incidence and mortality in China, 2022","volume":"46","author":"Zheng RS","year":"2024","journal-title":"Zhonghua Zhong Liu Za Zhi [Chin J Oncol]"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.979809"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/ijph.2024.1607245"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.17200"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e318254f90c"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-024-07721-w"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/08941939.2020.1850944"},{"key":"e_1_2_12_14_1","first-page":"532","article-title":"Discussion on cervical cytology in postmenopausal women","volume":"76","author":"Campaner AB","year":"2024","journal-title":"Minerva Obstet Gynecol"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-023-10888-1"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.03.008"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3728"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2009.02.011"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.10.7233-7235.1996"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2008.10.012"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0894-536"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.843671"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6250"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26326"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23519"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/IGC.0b013e3181c7fe6e"},{"key":"e_1_2_12_27_1","first-page":"1674","article-title":"Assessing methylation status of PAX1 in cervical scrapings, as a novel diagnostic and predictive biomarker, was closely related to screen cervical cancer","volume":"8","author":"Xu J","year":"2015","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-024-01731-w"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-024-13082-z"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-024-01804-w"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16071430"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1481626"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1159\/000477556"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2021.11.011"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmedr.2024.102679"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-2034"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/v9100267"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2015.3295"},{"key":"e_1_2_12_39_1","first-page":"5147","article-title":"Elderly women above screening age diagnosed with cervical cancer have a worse prognosis","volume":"34","author":"Darlin L","year":"2014","journal-title":"Anticancer Res"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-018-2617-5"},{"key":"e_1_2_12_41_1","first-page":"437","article-title":"Guidelines for cervical cancer screening in China","volume":"24","author":"Li M","year":"2023","journal-title":"Chin J Clin Obstet Gynecol"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.12718"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/jwh.2020.8849"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2021.02.017"},{"key":"e_1_2_12_45_1","volume-title":"WHO Guideline for Screening and Treatment of Cervical Pre\u2010Cancer Lesions for Cervical Cancer Prevention","author":"World Health Organization","year":"2021"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr2030640"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-018-4936-9"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djq001"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-03014-6"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-14-803"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.70245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T05:24:23Z","timestamp":1766035463000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.70245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,14]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,2,15]]}},"alternative-id":["10.1002\/ijc.70245"],"URL":"https:\/\/doi.org\/10.1002\/ijc.70245","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,14]]},"assertion":[{"value":"2025-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}