{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:25:52Z","timestamp":1773815152634,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["Institutional Strategic Support Fund (ISSF)"],"award-info":[{"award-number":["Institutional Strategic Support Fund (ISSF)"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2026,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Exercise protects against colon cancer progression, but the underlying biological mechanisms remain unclear. One proposed mechanism is the release of bioactive molecules into the systemic circulation during exercise, which may act directly on tumour cells to suppress DNA damage, inhibit proliferation, and preserve genomic stability. Here, we profiled the serum proteomic response to acute exercise and evaluated the effects of exercise\u2010conditioned human serum on DNA damage kinetics and transcriptomic signatures in colon cancer cells. Blood samples were collected from 30 overweight\/obese adults before and immediately after a maximal incremental cycling test. LoVo cells were exposed to pre\u2010 or post\u2010exercise serum, treated with 2\u2009Gy irradiation, and assessed for \u03b3\u2010H2AX foci over 24\u2009h. Acute exercise increased the relative abundance of 13 proteins in serum (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), including interleukin\u20106 (IL\u20106) and its soluble receptor IL\u20106R, reflecting systemic activation of acute\u2010phase immune and vascular signalling. Compared to pre\u2010exercise serum, post\u2010exercise serum significantly reduced \u03b3\u2010H2AX foci in LoVo cells at 6\u2009h (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.010) and decreased the area under the curve (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.014), indicating accelerated DNA repair. Post\u2010exercise serum also increased expression of the DNA repair gene\n                    <jats:italic>PNKP<\/jats:italic>\n                    , with and without irradiation (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.007 and\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.029, respectively). Transcriptomic analysis revealed upregulation of mitochondrial energy metabolism and downregulation of cell cycle and proteasome\u2010related pathways. These findings suggest that acute exercise elicits systemic responses that enhance DNA repair and shift colon cancer cells towards a less proliferative transcriptomic state under sublethal genotoxic stress, offering a potential mechanistic explanation for the protective effects of exercise against colorectal carcinogenesis.\n                  <\/jats:p>","DOI":"10.1002\/ijc.70271","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T03:03:03Z","timestamp":1765594983000},"page":"2641-2649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exercise serum promotes\n                    <scp>DNA<\/scp>\n                    damage repair and remodels gene expression in colon cancer cells"],"prefix":"10.1002","volume":"158","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4734-1446","authenticated-orcid":false,"given":"Samuel T.","family":"Orange","sequence":"first","affiliation":[{"name":"School of Biomedical, Nutritional and Sport Sciences, Faculty of Medical Sciences, Newcastle University  Newcastle upon Tyne UK"},{"name":"Emles Bioventures  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Dodd","sequence":"additional","affiliation":[{"name":"School of Biomedical, Nutritional and Sport Sciences, Faculty of Medical Sciences, Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharanya","family":"Nath","sequence":"additional","affiliation":[{"name":"School of Medicine and Public Health, University of Wisconsin  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Bowden","sequence":"additional","affiliation":[{"name":"School of Biomedical, Nutritional and Sport Sciences, Faculty of Medical Sciences, Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alastair R.","family":"Jordan","sequence":"additional","affiliation":[{"name":"School of Science, Technology and Health, York St John University  York UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Tweddle","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Hedley","sequence":"additional","affiliation":[{"name":"Bioinformatics Support Unit, Faculty of Medical Sciences, Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ifeoma","family":"Chukwuma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biosciences University of Nigeria  Enugu Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Hickson","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3591-653X","authenticated-orcid":false,"given":"Sweta Sharma","family":"Saha","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University  Newcastle upon Tyne UK"},{"name":"School of Health &amp; Life Sciences, Teesside University  Middlesbrough UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-8-316"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep43086"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2022-106445"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2502760"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2023.08.001"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djaa114"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2016-0153"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(24)00387-X"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jshs.2025.101036"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-16-0602"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14635"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33982"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0409-3"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1140735"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-017-0691-y"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122612899"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42834-x"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.1.185"},{"key":"e_1_2_12_20_1","first-page":"288","article-title":"Long 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