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This proof\u2010of\u2010concept study aimed to develop a robust\n                    <jats:italic>TP53<\/jats:italic>\n                    mutation panel for detecting HGSC through targeted DNA sequencing in both liquid and solid biopsies. We constructed a custom\n                    <jats:italic>TP53<\/jats:italic>\n                    gene panel and utilized a PCR\u2010based unique molecular identifier approach for next\u2010generation sequencing to analyze 94 samples from 11 patients diagnosed with HGSC, including primary tumor, plasma, ascites, ovarian cyst fluid, vaginal, endocervical and endometrial samples. Detected\n                    <jats:italic>TP53<\/jats:italic>\n                    mutations were analyzed, categorized, and their frequencies calculated. Pathogenic\n                    <jats:italic>TP53<\/jats:italic>\n                    mutations were identified in all patients, with 91% of the patients exhibiting one unique paired mutation across three or more sample types. The panel demonstrated high sensitivity and technical reproducibility, successfully detecting\n                    <jats:italic>TP53<\/jats:italic>\n                    mutations across all sample types, with as little as 2.6\u2009ng of DNA.\n                    <jats:italic>TP53<\/jats:italic>\n                    mutations were consistently found in ascites, ovarian cyst fluid, and plasma samples, confirming the presence of pathogenic mutations in each sample type across all patients. This study underscores the potential of liquid biopsies in clinical molecular diagnostics. The\n                    <jats:italic>TP53<\/jats:italic>\n                    mutation panel presented in this proof\u2010of\u2010concept study offers a promising approach for differential diagnostics and detection of HGSC, informative data prior to extended investigation and first\u2010line treatment guidance.\n                  <\/jats:p>","DOI":"10.1002\/ijc.70277","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T12:02:46Z","timestamp":1765281766000},"page":"2043-2050","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detecting\n                    <scp>\n                      <i>TP53<\/i>\n                    <\/scp>\n                    mutations in paired liquid and tissue biopsies from patients with high\u2010grade serous ovarian 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