{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:26:12Z","timestamp":1773815172430,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009053","name":"Wellcome Trust DBT India Alliance","doi-asserted-by":"publisher","award":["IA\/CPHE\/18\/1\/503946"],"award-info":[{"award-number":["IA\/CPHE\/18\/1\/503946"]}],"id":[{"id":"10.13039\/501100009053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2026,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Persistent infection with human papillomavirus (HPV) is associated with an increased risk of head and neck cancers (HNC), particularly oropharyngeal cancer. This longitudinal cohort study investigated the prevalence and dynamics of oral HPV infection among healthy adults in South India. A total of 5325 participants were enrolled, and demographic, behavioral, and oral gargle samples were collected. HPV deoxyribonucleic acid (DNA) was detected using nested PCR (PGMY\/GP) and genotyped by Sanger sequencing. HPV16 messenger ribonucleic acid (mRNA) expression was analyzed by reverse transcriptase polymerase chain reaction (RT\u2010PCR) and validated using droplet digital polymerase chain reaction (ddPCR). Logistic regression analyses were performed to estimate odds ratios. At baseline, 5011 samples were analyzed; 274 (5.5%) were HPV\u2010positive and 4737 were negative. Among 3792 initially HPV\u2010negative participants followed up, 126 (3.3%) acquired new infections (median 8\u2009months; range, 6\u201315\u2009months). Of 274 baseline HPV\u2010positive individuals, 241 were followed up: 24 (10%) showed persistence, while 217 (90%) cleared infection after a median of 9\u2009months (range, 6\u201320\u2009months). Sanger sequencing of 471 samples from prevalent and incident infections yielded 290 (61.6%) high\u2010quality sequences; 284 (98%) were HPV16, with isolated detections of HPV18, HPV66, HPV70, and HPV89. Ten novel variants were identified\u2014nine HPV16 and one HPV89\u2014while remaining sequences aligned with established Indian cervical HPV lineages. None of the 176 HPV16 DNA\u2010positive samples analyzed expressed mRNA by RT\u2010PCR, confirmed in 136 samples using ddPCR. Oral HPV infection among healthy individuals in India appears predominantly transient. Long\u2010term monitoring may elucidate the oncogenic potential of oral HPV in this population.<\/jats:p>","DOI":"10.1002\/ijc.70298","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T12:55:14Z","timestamp":1767099314000},"page":"2602-2612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence, incidence, and dynamics of oral human papillomavirus infection among healthy individuals\u2014A molecular epidemiology field study from India"],"prefix":"10.1002","volume":"158","author":[{"given":"Nandimandalam Venkata","family":"Vani","sequence":"first","affiliation":[{"name":"Department of Epidemiology, Biostatistics and Cancer Registry Cancer Institute (WIA)  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajendran","family":"Madhanagopal","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Biostatistics and Cancer Registry Cancer Institute (WIA)  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malliga J.","family":"Subramanian","sequence":"additional","affiliation":[{"name":"Department of Preventive Oncology Cancer Institute (WIA)  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirley","family":"Sundersingh","sequence":"additional","affiliation":[{"name":"Department of Oncopathology Cancer Institute (WIA)  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antony Xavier","family":"Helen","sequence":"additional","affiliation":[{"name":"Department of Oncopathology Cancer Institute (WIA)  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balaiah","family":"Meenakumari","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology Cancer Institute (WIA)  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deva Magendhra","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology Cancer Institute (WIA)  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4671-8099","authenticated-orcid":false,"given":"Trivadi Sundaram","family":"Ganesan","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Sri Ramachandra Institute of Higher Education and Research  Chennai India"},{"name":"Department of Medical Oncology and Clinical Research Laboratory for Cancer Biology, Cancer Institute (WIA)  Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5427-147X","authenticated-orcid":false,"given":"Rajaraman","family":"Swaminathan","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Biostatistics and Cancer Registry Cancer Institute (WIA)  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29210"},{"key":"e_1_2_11_4_1","unstructured":"BruniL AlberoG SerranoB et al.Human papillomavirus and related diseases in India. 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