{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:25:50Z","timestamp":1773815150274,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100021498","name":"San Francisco Arts Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100021498","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016913","name":"MSDAVENIR","doi-asserted-by":"publisher","award":["DS\u20102018\u20100007"],"award-info":[{"award-number":["DS\u20102018\u20100007"]}],"id":[{"id":"10.13039\/100016913","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2026,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Minimal residual disease (MRD) assessment using circulating nuclear DNA (cir\u2010nDNA) testing has demonstrated strong prognostic value in patients with operable stage III colon cancer (CC). However, further clinical research is needed to optimize the use of adjuvant therapy in this context. Since the sensitivity of variant allele frequency (VAF)\u2010based MRD detection depends on total cirDNA concentration, we investigated its variation in 67 stage III CC patients over 8\u2009weeks following surgery. A majority of patients showed significantly higher post\u2010surgical cir\u2010nDNA levels compared to their pre\u2010surgical baseline\u201471.1% during the first month and 51.4% during the second. Cir\u2010nDNA levels tended to considerably vary (up to 18\u2010fold), especially during the first 3\u2009weeks after surgery. We also observed a strong correlation between cir\u2010nDNA levels and neutrophil extracellular traps (NETs) markers throughout the 2\u2010month post\u2010operative period. While previous studies have generally assumed that cir\u2010nDNA levels decrease significantly within 1\u2009month post\u2010surgery in MRD\u2010negative stage III patients, our findings challenge this paradigm. NETs appear to be a substantial source of cir\u2010nDNA and represent a major confounding factor in interpreting total cir\u2010nDNA, given the high inter\u2010individual variability in NETs production after surgery. Our data suggest that defining a single optimal time point for MRD testing may be misleading. Instead, we propose a sampling window between fourth and sixth week post\u2010surgery. Additionally, our results call into question the reliance on VAF as a standalone metric for MRD detection. The most robust strategy would involve integrated monitoring of total cir\u2010nDNA concentration, absolute mutant DNA levels, and NETs\u2010associated inflammation.<\/jats:p>","DOI":"10.1002\/ijc.70370","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:06:33Z","timestamp":1770818793000},"page":"2771-2783","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Post\u2010surgery level of circulating\n                    <scp>DNA<\/scp>\n                    in stage\n                    <scp>III<\/scp>\n                    colon cancer patients: Impact on the reliability of minimal residual disease detection"],"prefix":"10.1002","volume":"158","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0537-1460","authenticated-orcid":false,"given":"Andrei","family":"Kudriavtsev","sequence":"first","affiliation":[{"name":"IRCM Montpellier Cancer Research Institute, INSERM U1194, Montpellier University  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saidi","family":"Daoud","sequence":"additional","affiliation":[{"name":"IRCM Montpellier Cancer Research Institute, INSERM U1194, Montpellier University  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catalina Isabel Cofre","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Medical Oncology Department Institut Catal\u00e0 d'Oncologia (ICO)\u2014IDIBELL  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexia","family":"Mirandola","sequence":"additional","affiliation":[{"name":"IRCM Montpellier Cancer Research Institute, INSERM U1194, Montpellier University  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekaterina","family":"Pisareva","sequence":"additional","affiliation":[{"name":"IRCM Montpellier Cancer Research Institute, INSERM U1194, Montpellier University  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier Gonzalo","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Medical Oncology Department VHIO Vall d'Hebron Institute of Oncology  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Macagno","sequence":"additional","affiliation":[{"name":"Istituto di Candiolo Fondazione del Piemonte per l'Oncologia\u2014IRCCS  Candiolo Torino Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia Saoudi","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Medical Oncology Department VHIO Vall d'Hebron Institute of Oncology  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyne","family":"Crapez","sequence":"additional","affiliation":[{"name":"IRCM Montpellier Cancer Research Institute, INSERM U1194, Montpellier University  Montpellier France"},{"name":"ICM Institut R\u00e9gional du Cancer de Montpellier  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Ychou","sequence":"additional","affiliation":[{"name":"IRCM Montpellier Cancer Research Institute, INSERM U1194, Montpellier University  Montpellier France"},{"name":"ICM Institut R\u00e9gional du Cancer de Montpellier  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramon Salazar","family":"Soler","sequence":"additional","affiliation":[{"name":"Medical Oncology Department Institut Catal\u00e0 d'Oncologia (ICO)\u2014IDIBELL  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabetta","family":"Fenocchio","sequence":"additional","affiliation":[{"name":"Istituto di Candiolo Fondazione del Piemonte per l'Oncologia\u2014IRCCS  Candiolo Torino Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula X. Fernandez","family":"Calotti","sequence":"additional","affiliation":[{"name":"Medical Oncology Department Institut Catal\u00e0 d'Oncologia (ICO)\u2014IDIBELL  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thibault","family":"Mazard","sequence":"additional","affiliation":[{"name":"IRCM Montpellier Cancer Research Institute, INSERM U1194, Montpellier University  Montpellier France"},{"name":"ICM Institut R\u00e9gional du Cancer de Montpellier  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina Santos","family":"Vivas","sequence":"additional","affiliation":[{"name":"Medical Oncology Department Institut Catal\u00e0 d'Oncologia (ICO)\u2014IDIBELL  Barcelona Spain"},{"name":"Faculty of Medicine and Health Sciences Universitat de Barcelona CIBERONC  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Elez","sequence":"additional","affiliation":[{"name":"Medical Oncology Department VHIO Vall d'Hebron Institute of Oncology  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Di Nicolantonio","sequence":"additional","affiliation":[{"name":"Istituto di Candiolo Fondazione del Piemonte per l'Oncologia\u2014IRCCS  Candiolo Torino Italy"},{"name":"Department of Oncology University of Torino  Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain R.","family":"Thierry","sequence":"additional","affiliation":[{"name":"IRCM Montpellier Cancer Research Institute, INSERM U1194, Montpellier University  Montpellier France"},{"name":"ICM Institut R\u00e9gional du Cancer de Montpellier  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2024.114118"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(20)30527-1"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2024.102735"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2023.2274123"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-21-2181"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jncc.2024.01.003"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-020-02227-2"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3511"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv177"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.103826"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-022-01125-8"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.05.022"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2018.298323"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28106"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-41593-4"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201901917RR"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2023.1104732"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2014.02.005"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507904102"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12827"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12729"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2023.41.4_suppl.5"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.105352"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-022-02217-w"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aag1711"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16122276"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2023.03.008"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.105"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.29887"},{"key":"e_1_2_12_31_1","article-title":"Association of the immediate perioperative dynamics of circulating DNA levels and neutrophil extracellular traps formation in cancer patients. Precis","volume":"7","author":"Kudriavtsev A","year":"2024","journal-title":"Clin Med"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.02.013"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan2415"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.11.050"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07466-6"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1200\/PO.22.00694"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-23-0824"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-21-1252"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.70370","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ijc.70370","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.70370","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:33:11Z","timestamp":1773811991000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.70370"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,11]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,5,15]]}},"alternative-id":["10.1002\/ijc.70370"],"URL":"https:\/\/doi.org\/10.1002\/ijc.70370","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,11]]},"assertion":[{"value":"2025-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}