{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T19:52:20Z","timestamp":1783453940114,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001317","name":"Swansea University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001317","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009828","name":"Cancer Research Wales","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009828","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We investigated mechanisms underlying circulating DNA damage across the gastro\u2010oesophageal reflux disease (GORD), Barrett's oesophagus (BO) and oesophageal adenocarcinoma (OAC) spectrum using the lymphocyte micronucleus (MN) assay. MN frequency (MN%) was quantified in lymphocytes from healthy volunteers (HVs), GORD, BO and OAC patients, alongside plasma biomarkers of inflammation and oxidative stress. Ex\u00a0vivo challenge assays assessed lymphocyte responses to hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ), vinblastine and the bile acid deoxycholic acid (DCA). Tissue\u2010based NF\u2010\u03baB expression was also correlated with MN levels. OAC patients exhibited significantly elevated MN% compared with HVs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), GORD (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) and BO (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.016). OAC lymphocytes showed increased sensitivity to vinblastine relative to HVs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.025) and GORD patients (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.033). Higher baseline MN% correlated with reduced inducible MN formation following H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    or DCA, suggesting altered oxidative stress responses. MN% also associated with plasma 8\u2010OHdG, IL\u20108 and 2\u20323\u2032\u2010cGAMP, and with reduced oesophageal tissue I\u03baB, indicating activation of oxidative and cGAS\u2010STING\/NF\u2010\u03baB signalling. These findings highlight systemic aneugenic and oxidative stress processes that contribute to lymphocyte MN formation in OAC and suggest that MN% may serve as a minimally invasive indicator of inflammation\u2010linked genomic instability. Further investigation is needed to determine its relevance to OAC progression.\n                  <\/jats:p>","DOI":"10.1002\/ijc.70494","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:05:02Z","timestamp":1777100702000},"page":"1311-1321","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lymphocyte Micronucleus Formation Is Driven by Inflammation\u2010Induced Oxidative\n                    <scp>DNA<\/scp>\n                    Damage in Oesophageal Cancer Development"],"prefix":"10.1002","volume":"159","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0960-6328","authenticated-orcid":false,"given":"Kathryn","family":"Munn","sequence":"first","affiliation":[{"name":"In Vitro Toxicology Group Swansea University Medical School, Swansea University  Swansea 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