{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T01:44:25Z","timestamp":1788831865683,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100018846","name":"Erik og Susanna Olesens Almenvelg\u00f8rende Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018846","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010105","name":"Savvaerksejer Jeppe Juhl og Hustru Ovita Juhls Mindelegat","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010105","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008363","name":"Kr\u00e6ftens Bek\u00e6mpelse","doi-asserted-by":"publisher","award":["R278\u2010A16626"],"award-info":[{"award-number":["R278\u2010A16626"]}],"id":[{"id":"10.13039\/100008363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Penile cancer is a severe disease, and a large proportion is related to high\u2010risk (hr) human papillomavirus (HPV). P16 is used as an immunohistochemical marker of HPV in clinical diagnostics. Population\u2010based data on the prevalence of p16 in penile cancer is scarce. We investigated the prevalence of p16 positivity, and p16 in combination with hrHPV DNA, in a large population of Danish penile squamous cell carcinomas (SCC). Cases diagnosed during 1995\u20132020 were identified in the Danish Pathology Databank, and tumor samples were tested for HPV DNA (INNO\u2010LiPA assay). After immunohistochemical p16\u2010staining, the slides were evaluated by expert pathologists. p16 prevalence was calculated and the combined p16 and hrHPV DNA status (double positivity, double negativity, p16 positive only, and hrHPV DNA positive only) was assessed according to calendar time and histology. Time trends were assessed by calculating estimated annual percentage changes. Of 1028 penile SCCs, 42.8% were p16 positive and 37.8% were double positive. We found no significant changes in prevalences over the study period. The highest prevalence of double positivity was observed in SCCs with HPV\u2010associated histological features, especially basaloid (63.6%\u201387.5%). hrHPV genotypes covered by the 9\u2010valent HPV\u2010vaccine (HPV16\/18\/31\/33\/45\/52\/58) were detected in 97.9% of double positive SCCs. Based on this large population\u2010based study, we demonstrate a good correlation between p16 and hrHPV DNA and no substantial changes in the prevalences over time (1995\u20132020). Nearly all double positive SCCs harbored a hrHPV genotype that could potentially be prevented by vaccination, pointing to a likely future decrease in penile cancer in Denmark.<\/jats:p>","DOI":"10.1002\/ijc.70736","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T13:16:33Z","timestamp":1788786993000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["p16 Overexpression and Human Papillomavirus Positivity in More Than 1000 Penile Cancers"],"prefix":"10.1002","author":[{"given":"Marianne Gardar","family":"St\u00e6rk","sequence":"first","affiliation":[{"name":"Unit of Virus, Lifestyle and Genes Danish Cancer Institute  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitte Gr\u00f8nk\u00e6r","family":"Toft","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital Rigshospitalet  Copenhagen Denmark"},{"name":"Department of Clinical Medicine University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Stilling","sequence":"additional","affiliation":[{"name":"Department of Pathology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Elversang","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne","family":"Kj\u00e6r\u2010Frifeldt","sequence":"additional","affiliation":[{"name":"Department of Pathology Vejle Hospital, Lillebaelt Hospital  Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja H\u00f6hle","family":"Hjulskov","sequence":"additional","affiliation":[{"name":"Department of Pathology Aalborg University Hospital  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Stifter\u2010Vretenar","sequence":"additional","affiliation":[{"name":"Department of Pathology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Aarslev","family":"Schou","sequence":"additional","affiliation":[{"name":"Department of Pathology Vejle Hospital, Lillebaelt Hospital  Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Aagaard","sequence":"additional","affiliation":[{"name":"Department of Urology Copenhagen University Hospital Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Meidahl","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Oncology Copenhagen University Hospital Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob Kristian","family":"Jakobsen","sequence":"additional","affiliation":[{"name":"Department of Urology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Clinical Medicine Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Munk","sequence":"additional","affiliation":[{"name":"Unit of Virus, Lifestyle and Genes Danish Cancer Institute  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8347-1398","authenticated-orcid":false,"given":"Susanne Kr\u00fcger","family":"Kj\u00e6r","sequence":"additional","affiliation":[{"name":"Unit of Virus, Lifestyle and Genes Danish Cancer Institute  Copenhagen Denmark"},{"name":"Department of Clinical Medicine University of Copenhagen  Copenhagen Denmark"},{"name":"Department of Gynecology Copenhagen University Hospital Rigshospitalet  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"\u201cS\u00f8ren Friis og Simon Mathis K Kr\u00e6ftepidemiologi og Monitorering Center for Kr\u00e6ftforskning Kr\u00e6ftens bek\u00e6mpelse. 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