{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:51:03Z","timestamp":1791568263381,"version":"4.3.4"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl Journal of Cancer"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    SACC\u201083 was established in 1990 from a patient with adenoid cystic carcinoma, with SACC\u2010LM as its lung\u2010metastatic subline derived through in\u00a0vivo selection in nude mice. Although several other purported ACC cell lines have already been reported as cross\u2010contaminated, the true origin of SACC\u201083 and SACC\u2010LM has remained unclear despite their wide use in the field. To address this, we carried out short tandem repeat (STR) profiling on 10 SACC\u201083 and SACC\u2010LM samples submitted from six independent laboratories across China during our routine authentication work. Statistical analysis using the classic Tanabe comparison algorithm on the CLASTR website returned matches to multiple common cell line contaminants. Among these, comparison with HeLa yielded a random match probability (RMP) of 1.29\u2009\u00d7\u200910\n                    <jats:sup>\u221219<\/jats:sup>\n                    and a likelihood ratio (LR) of 7.75\u2009\u00d7\u200910\n                    <jats:sup>18<\/jats:sup>\n                    . These findings provide direct evidence that both SACC\u201083 and SACC\u2010LM are HeLa\u2010derived contaminants rather than authentic SACC models. We therefore recommend that researchers suspend further use of these two lines and highlight the broader role that independent authentication platforms, by virtue of their direct access to cell line data, can play as an effective early\u2010warning system against misidentification in the scientific community.\n                  <\/jats:p>","DOI":"10.1002\/ijc.70771","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:01:36Z","timestamp":1791565296000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cell Line Authentication Reveals\n                    <scp>HeLa<\/scp>\n                    \u2010Derived Contamination of\n                    <scp>SACC<\/scp>\n                    \u201083 and\n                    <scp>SACC<\/scp>\n                    \u2010\n                    <scp>LM<\/scp>\n                    in Adenoid Cystic Carcinoma Research"],"prefix":"10.1002","author":[{"given":"Cuijie","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine Suzhou Medical College of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiankun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Division of DNA Typing Genetic Testing Biotechnology (Suzhou) Co., Ltd.  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuekun","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of DNA Typing Genetic Testing Biotechnology (Suzhou) Co., Ltd.  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5907-8062","authenticated-orcid":false,"given":"Yuzhen","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine Suzhou Medical College of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"29","article-title":"Establishment of a Human Cancer Cell Line From Adenoid Cystic Carcinoma of the Minor Salivary Gland","volume":"25","author":"Li S.","year":"1990","journal-title":"Zhonghua Kou Qiang Yi Xue Za Zhi"},{"issue":"1","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.3892\/or.2013.2460","article-title":"Molecular Characteristics of Homologous Salivary Adenoid Cystic Carcinoma Cell Lines With Different Lung Metastasis Ability","volume":"30","author":"Wang L.","year":"2013","journal-title":"Oncology Reports"},{"issue":"6","key":"e_1_2_10_4_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0006040","article-title":"Genetic Profiling Reveals Cross\u2010Contamination and Misidentification of 6 Adenoid Cystic Carcinoma Cell Lines: ACC2, ACC3, ACCM, ACCNS, ACCS and CAC2","volume":"4","author":"Phuchareon J.","year":"2009","journal-title":"PLoS One"},{"issue":"1","key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/ijc.25242","article-title":"Check Your Cultures! A List of Cross\u2010Contaminated or Misidentified Cell Lines","volume":"127","author":"Capes\u2010Davis A.","year":"2010","journal-title":"International Journal of Cancer"},{"issue":"4493","key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1126\/science.6451928","article-title":"Cross\u2010Contamination of Cells in Culture","volume":"212","author":"Nelson\u2010Rees W. A.","year":"1981","journal-title":"Science"},{"key":"e_1_2_10_7_1","unstructured":"\u201cANSI\/ATCC ASN\u20100002\u20102022 Authentication of Human Cell Lines: Standardization of STR Profiling American National Standards Institute \u201d 2022."},{"key":"e_1_2_10_8_1","volume-title":"Analysis and Application of Human DNA Fluorescent STR Typing Results GA\/T 1163\u20102014","author":"Ministry of Public Security of the People's Republic of China","year":"2014"},{"issue":"5","key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1002\/ijc.32639","article-title":"CLASTR: The Cellosaurus STR Similarity Search Tool\u2014A Precious Help for Cell Line Authentication","volume":"146","author":"Robin T.","year":"2020","journal-title":"International Journal of Cancer"},{"issue":"2","key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.7171\/jbt.18-2902-002","article-title":"The Cellosaurus, a Cell\u2010Line Knowledge Resource","volume":"29","author":"Bairoch A.","year":"2018","journal-title":"Journal of Biomolecular Techniques"},{"issue":"11","key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"2510","DOI":"10.1002\/ijc.27931","article-title":"Match Criteria for Human Cell Line Authentication: Where Do We Draw the Line?","volume":"132","author":"Capes\u2010Davis A.","year":"2013","journal-title":"International Journal of Cancer"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.fsigen.2016.06.008","article-title":"Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on Quality Control of Autosomal Short Tandem Repeat Allele Frequency Databasing (STRidER)","volume":"24","author":"Bodner M.","year":"2016","journal-title":"Forensic Science International: Genetics"},{"issue":"1","key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/ijc.23233","article-title":"Persistent Use of \u2018False\u2019 Cell Lines","volume":"122","author":"Lacroix M.","year":"2008","journal-title":"International Journal of Cancer"},{"key":"e_1_2_10_14_1","volume-title":"ENFSI Guideline for Evaluative Reporting in Forensic Science. Strengthening the Evaluation of Forensic Results Across Europe","author":"ENFSI","year":"2015"},{"issue":"1","key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/ijc.34677","article-title":"Multiple Approaches Revealed MGc80\u20103 as a Somatic Hybrid With HeLa Cells Rather Than a Gastric Cancer Cell Line","volume":"154","author":"Cao F.","year":"2024","journal-title":"International Journal of Cancer"},{"issue":"2","key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1007\/s13577-023-01011-4","article-title":"Investigation of the Mixed Origins of the MGC\u2010803 Cell Line Reveals That It Is a Hybrid Cell Line Derived From HeLa","volume":"37","author":"Yang M.","year":"2024","journal-title":"Human Cell"},{"issue":"12","key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1038\/s41416-024-02675-x","article-title":"HeLa Cells Cross\u2010Contaminated Nasopharyngeal Carcinoma Cell Lines: Still a Common Problem","volume":"130","author":"Makowska A.","year":"2024","journal-title":"British Journal of Cancer"},{"issue":"18","key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"10696","DOI":"10.1128\/JVI.01457-14","article-title":"Comprehensive High\u2010Throughput RNA Sequencing Analysis Reveals Contamination of Multiple Nasopharyngeal Carcinoma Cell Lines With HeLa Cell Genomes","volume":"88","author":"Strong M. 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A.","year":"1999","journal-title":"International Journal of Cancer"},{"key":"e_1_2_10_21_1","volume-title":"2\u2010D Cell Cultures","author":"ACCRF","year":"2025"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.70771","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ijc.70771","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.70771","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:01:41Z","timestamp":1791565301000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.70771"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,9]]},"references-count":20,"alternative-id":["10.1002\/ijc.70771"],"URL":"https:\/\/doi.org\/10.1002\/ijc.70771","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,9]]},"assertion":[{"value":"2026-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ijc.70771"}}