{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T16:50:39Z","timestamp":1771951839795,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T00:00:00Z","timestamp":1587600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fluid Research Grant"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J Gynecology & Obste"],"published-print":{"date-parts":[[2020,7]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>To evaluate a treponemal enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>) as an alternative screening test for syphilis in pregnant women.<\/jats:p><\/jats:sec>Methods<\/jats:title>A cross\u2010sectional study of diagnostic test accuracy was carried out in a large volume laboratory from a tertiary care center. A total of 416 serum samples, including 102 archived syphilis Treponema pallidum<\/jats:italic> hemagglutination (TPHA<\/jats:styled-content>)\u2010positive samples and 314 samples from pregnant women, were used to determine the sensitivity and specificity of ELISA<\/jats:styled-content>. All the samples were subjected to Venereal Disease Research Laboratory (VDRL<\/jats:styled-content>), rapid plasma reagin (RPR<\/jats:styled-content>), ELISA<\/jats:styled-content>, and TPHA<\/jats:styled-content> tests. Performance characteristics of VDRL<\/jats:styled-content>, RPR<\/jats:styled-content>, and ELISA<\/jats:styled-content> were calculated with TPHA<\/jats:styled-content> as a reference standard test.<\/jats:p><\/jats:sec>Results<\/jats:title>VDRL<\/jats:styled-content> and RPR<\/jats:styled-content> exhibited higher false positivity of 10.5% and 9.6%, respectively, compared to 2.5% by ELISA<\/jats:styled-content>. The sensitivity and specificity of ELISA<\/jats:styled-content> were 98% and 97.5%, of VDRL<\/jats:styled-content> were 71.6% and 89.5%, and of RPR<\/jats:styled-content> were 73.5% and 90.5%, respectively. Moreover, ELISA<\/jats:styled-content> had an excellent agreement (kappa=0.9) with TPHA<\/jats:styled-content> compared to VDRL<\/jats:styled-content>\/RPR<\/jats:styled-content> which had a moderate agreement (kappa=0.6) only.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>ELISA<\/jats:styled-content> has the potential to replace VDRL<\/jats:styled-content>\/RPR<\/jats:styled-content> as a screening test for syphilis in centers that can perform ELISA<\/jats:styled-content>, especially for antenatal screening.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ijgo.13154","type":"journal-article","created":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T14:16:11Z","timestamp":1586009771000},"page":"103-107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Enzyme\u2010linked immunosorbent assay (ELISA<\/scp>) versus Venereal Disease Research Laboratory test (VDRL<\/scp>) and rapid plasma reagin test (RPR<\/scp>) for screening of syphilis in pregnant women"],"prefix":"10.1002","volume":"150","author":[{"given":"Dhanalakshmi","family":"Solaimalai","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Swati","family":"Rathore","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manisha M.","family":"Beck","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annie","family":"Regi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binesh L.","family":"Yesudhason","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balaji","family":"Veeraraghavan","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.J.","family":"Prakash","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology Christian Medical College Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.1.1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2013-003347"},{"key":"e_1_2_9_4_1","unstructured":"Sexually transmitted infections (STIs)[Internet]. Available from:https:\/\/www.who.int\/news-room\/fact-sheet\u200bs\/detai\u200bl\/sexua\u200blly-trans\u200bmitted-infec\u200btions-(stis). Accessed August 22 2019."},{"key":"e_1_2_9_5_1","first-page":"202","article-title":"Audit of management of pregnant women with positive VDRL tests","volume":"14","author":"Mathai E","year":"2001","journal-title":"Natl Med J India"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.12336"},{"key":"e_1_2_9_7_1","unstructured":"World Health Organization.WHO guideline on syphilis screening and treatment for pregnant women[Internet].2017.http:\/\/www.ncbi.nlm.nih.gov\/books\/\u200bNBK49\u200b9742\/. 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