{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T04:43:50Z","timestamp":1786769030101,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J Gynecology &amp; Obste"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify whether the transfer of blastocysts that have been vitrified, thawed, biopsied, revitrified, and subsequently rethawed affects clinical outcome and neonatal outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A retrospective study was conducted in a single assisted reproduction technology center from September 2016 to March 2021. Women undergoing single frozen euploid blastocysts transfer were stratified into two groups based on number of vitrification\u2010thawing cycles: single vitrification coupled with single biopsy (group A, <jats:italic>n<\/jats:italic>\u00a0=\u00a0177) and double vitrification coupled with single biopsy (group B, <jats:italic>n<\/jats:italic>\u00a0=\u00a030). Pregnancy and perinatal outcomes of the two groups were compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Clinical pregnancy rates were similar between the two groups. Group B was associated with a lower chance of live birth when compared with group A by different multivariable analysis models (model 1: odds ratio, 0.42 [95% confidence interval, 0.18\u20130.97], <jats:italic>P<\/jats:italic>\u00a0=\u00a00.041; model 2: odds ratio, 0.38 [95% confidence interval, 0.16\u20130.92], <jats:italic>P<\/jats:italic>\u00a0=\u00a00.033). No major obstetrical complication was reported in the two groups and only one malformation live birth was reported in group A.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The procedure of double vitrification\u2010warming cycles, coupled with single biopsy, increases pregnancy loss and ultimately diminishes live birth but does not affect perinatal outcome. Future studies with a larger sample size would help to validate the results.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ijgo.14355","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T04:13:34Z","timestamp":1658117614000},"page":"806-813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Double vitrification\u2013warming cycles, coupled with blastocyst biopsy, impair live birth but do not affect neonatal outcomes"],"prefix":"10.1002","volume":"160","author":[{"given":"Xiaofang","family":"Li","sequence":"first","affiliation":[{"name":"Assisted Reproduction Center, Northwest Women's and Children's Hospital  Xi'an People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Assisted Reproduction Center, Northwest Women's and Children's Hospital  Xi'an People's Republic of 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