{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T04:49:56Z","timestamp":1747284596858},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J Gynecology &amp; Obste"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The purpose of this preliminary investigation into the pathogenesis of pre\u2010eclampsia was to screen the differential proteins in the serum of pregnant women with normal pregnancy and early\u2010onset pre\u2010eclampsia using isobaric tags for relative and absolute quantitation (iTRAQ), so as to identify serum biomarkers for the early diagnosis of pre\u2010eclampsia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined the peripheral serum of 58 normal pregnant women and 42 pregnant women with early\u2010onset pre\u2010eclampsia using iTRAQ; the differentially expressed proteins were screened for bioinformatics analysis; and the expression of candidate proteins human leukocyte antigen\u20101 (HLA\u20101) and \u03b22\u2010microglobulin (\u03b22M) in placental tissues was detected using western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified a total of 63 differential proteins in the serum of patients from the normal control group and the pre\u2010eclampsia group, and this included 24 up\u2010regulated proteins and 39 down\u2010regulated proteins. The western blot results of placental tissue showed reduced HLA\u20101 expression (1.12\u2009\u00b1\u20090.23) in the placenta in the pre\u2010eclampsia group as compared with the normal control group (1.34\u2009\u00b1\u20090.22). Consistent with the results observed in the serum, \u03b22M in the placenta in the pre\u2010eclampsia group was significantly elevated (1.05\u2009\u00b1\u20090.47) in comparison with the normal group (0.75\u2009\u00b1\u20090.33) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this study, we found that iTRAQ technology was useful for identifying differentially expressed proteins in the peripheral serum of pregnant women with pre\u2010eclampsia, and that HLA\u20101 and \u03b22M, which may be involved in the occurrence of pre\u2010eclampsia, show promise as predictive markers of pre\u2010eclampsia.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ijgo.15312","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T09:05:23Z","timestamp":1703667923000},"page":"1072-1084","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of serum major histocompatibility complex I (<scp>HLA<\/scp>\u20101) and \u03b22\u2010microglobulin (<scp>\u03b22M<\/scp>) in pre\u2010eclampsia using isobaric tags for relative and absolute quantitation (<scp>iTRAQ<\/scp>)"],"prefix":"10.1002","volume":"165","author":[{"given":"Xu\u2010Feng","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Obstetrics Henan Provincial People's Hospital  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Henan Provincial People's Hospital  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Henan Provincial People's Hospital  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Henan Provincial People's Hospital  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan\u2010Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Henan Provincial People's Hospital  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010Ying","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics Henan Provincial People's Hospital  Zhengzhou Henan 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