{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T22:35:13Z","timestamp":1791758113233,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J Gynecology & Obste"],"published-print":{"date-parts":[[2024,9]]},"abstract":"Abstract<\/jats:title>Objectives<\/jats:title>Studies have investigated miR<\/jats:italic>\u2010125a for its predictable role in recurrent pregnancy loss (RPL) cases to regulate many biological events required for the maintenance of pregnancy by regulating its confirmed target genes LIFR<\/jats:italic>, ERBB2<\/jats:italic> and STAT3<\/jats:italic>.<\/jats:p><\/jats:sec>Methods<\/jats:title>The present study included 40 cases of women with at least two RPLs in \u226420\u2009weeks of gestation against 40 healthy multiparous women without a previous history of abortion. Expression analysis of ERBB2, LIFR, STAT3<\/jats:italic> and miR<\/jats:italic>\u2010125a was conducted by quantitative real\u2010time PCR (qPCR).<\/jats:p><\/jats:sec>Results<\/jats:title>The expression of miR<\/jats:italic>\u2010125a was significantly lower in the plasma of RPL cases (P<\/jats:italic>\u2009=\u20090.0001) and showed a significantly increased mean expression level in product of conception (2.56\u2010fold, P<\/jats:italic>\u2009<\u20090.0001). Among the target gene of miR<\/jats:italic>\u2010125a, ERBB2<\/jats:italic> and STAT3<\/jats:italic> gene expression level was significantly increased (2.58\u2010fold, P<\/jats:italic>\u2009=\u20090.04; 1.87\u2010fold, P<\/jats:italic>\u2009=\u20090.025), respectively in RPL cases while the LIFR<\/jats:italic> gene revealed comparable expression (P<\/jats:italic>\u2009=\u20090.64). Furthermore, expression analysis of ERBB2<\/jats:italic> gene with respect to its regulatory miR<\/jats:italic>\u2010125a cases depicted a significant association (P<\/jats:italic>\u2009=\u20090.0005). Kaplan\u2013Meier survival analysis revealed cases with low miR<\/jats:italic>\u2010125a expression had significantly shorter time to miscarriages, (log\u2010rank P<\/jats:italic>\u2009=\u20090.02). Also, decreased expression of miR<\/jats:italic>\u2010125a significantly conferred >2\u2010fold increased risk for RPL (HR\u2009=\u20092.34: P<\/jats:italic>\u2009<\u20090.05).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The overall conclusion of the study was that altered miR<\/jats:italic>\u2010125a expression may cause deregulation in target genes LIFR<\/jats:italic>, ERBB2<\/jats:italic> and STAT3<\/jats:italic> resulting in adverse consequence in the outcome of pregnancy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ijgo.15496","type":"journal-article","created":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T06:56:20Z","timestamp":1711436180000},"page":"1285-1296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Regulatory role of miR<\/i><\/scp>\u2010125a expression with respect to its target genes LIFR<\/i><\/scp>, ERBB2<\/i><\/scp> and STAT3<\/i><\/scp> in the pathogenesis of recurrent pregnancy losses"],"prefix":"10.1002","volume":"166","author":[{"given":"Usma","family":"Manzoor","sequence":"first","affiliation":[{"name":"Advanced Center for Human Genetics Sher\u2010I\u2010Kashmir Institute of Medical Sciences (SKIMS) Srinagar Jammu and Kashmir India"},{"name":"School of Life and Basic Sciences Jaipur National University Jaipur Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7912-2638","authenticated-orcid":false,"given":"Arshad A.","family":"Pandith","sequence":"additional","affiliation":[{"name":"Advanced Center for Human Genetics Sher\u2010I\u2010Kashmir Institute of Medical Sciences (SKIMS) Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"Amin","sequence":"additional","affiliation":[{"name":"Advanced Center for Human Genetics Sher\u2010I\u2010Kashmir Institute of Medical Sciences (SKIMS) Srinagar Jammu and Kashmir India"},{"name":"Department of Clinical Biochemistry University of Kashmir Srinagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima","family":"Wani","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology SKIMS Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahid M.","family":"Baba","sequence":"additional","affiliation":[{"name":"Advanced Center for Human Genetics Sher\u2010I\u2010Kashmir Institute of Medical Sciences (SKIMS) Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umer M.","family":"Wani","sequence":"additional","affiliation":[{"name":"Department of Biotechnology University of Kashmir Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheikh","family":"Mansoor","sequence":"additional","affiliation":[{"name":"Advanced Center for Human Genetics Sher\u2010I\u2010Kashmir Institute of Medical Sciences (SKIMS) Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qurat Ul","family":"Aein","sequence":"additional","affiliation":[{"name":"Advanced Center for Human Genetics Sher\u2010I\u2010Kashmir Institute of Medical Sciences (SKIMS) Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqra","family":"Anwar","sequence":"additional","affiliation":[{"name":"Advanced Center for Human Genetics Sher\u2010I\u2010Kashmir Institute of Medical Sciences (SKIMS) Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barjista","family":"Bahar","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology SKIMS Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aabid M.","family":"Koul","sequence":"additional","affiliation":[{"name":"Advanced Center for Human Genetics Sher\u2010I\u2010Kashmir Institute of Medical Sciences (SKIMS) Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dheera","family":"Sanadhya","sequence":"additional","affiliation":[{"name":"School of Life and Basic Sciences Jaipur National University Jaipur Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abida","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology SKIMS Srinagar Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,25]]},"reference":[{"issue":"78","key":"e_1_2_11_2_1","first-page":"179","article-title":"ACOG practice bulletin. 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