{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:14:08Z","timestamp":1792552448030,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T00:00:00Z","timestamp":1713744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J Gynecology &amp; Obste"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Tubal ectopic pregnancy (EP) is a leading cause of maternal morbidity and mortality. Studies have suggested that infection\u2010induced inflammatory responses are major risk factors for EP. The aim of the present study was to find an association between MMP2 and CD63 gene variants and risk of EP during <jats:italic>Chlamydia trachomatis<\/jats:italic> infection in an Indian population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fallopian tube samples of 120 EP and 120 tubal ligation women were collected. <jats:italic>C. trachomatis<\/jats:italic> was detected by PCR. The genotyping of MMP2 (rs17859882 G\/T, rs7201A\/C) and CD63(rs2231464 C\/T, rs376086542 A\/G) gene variants was done by qualitative real\u2010time PCR using allelic discrimination method (VIC\u2010 and FAM\u2010labeled).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequency of GG or GT genotype of MMP2 G\/T polymorphism (rs17859882) was 66.6% in infected EP and 36.7% in uninfected EP and 22% in tubal ligation controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001), while the frequency of AC or CC genotype of MMP2 A\/C polymorphism (rs7201) was 66.6% in infected EP and 20.6% in uninfected EP and 13.5% in tubal ligation controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). The frequency of CT or TT genotype of CD63 C\/T polymorphism (rs2231464) was 74% in infected EP and 21.8% in uninfected EP and 11.8% tubal ligation controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001), while the frequency of AG or GG genotype of CD63 A\/G polymorphism (rs376086542) was 48.1% in infected EP and 41.3% in uninfected EP and 18.6% tubal ligation controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study revealed a strong association between the presence of gene variants MMP2 (rs17859882 G\/T, rs7201A\/C) and CD63 (rs2231464 C\/T, rs376086542 A\/G) and risk of tubal EP during <jats:italic>C. trachomatis<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ijgo.15547","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T05:53:58Z","timestamp":1713851638000},"page":"99-106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Screening of single nucleotide polymorphism in matrix metalloproteinase\u20102 (<scp>MMP2<\/scp>) and tetraspanin <scp>CD63<\/scp> genes in <i>Chlamydia trachomatis<\/i>\u2010infected tubal ectopic pregnancy patients"],"prefix":"10.1002","volume":"166","author":[{"given":"Shipra","family":"Pant","sequence":"first","affiliation":[{"name":"Molecular Microbiology Laboratory ICMR\u2010National Institute of Pathology, Sriramachari Bhawan, Safdarjung Hospital Campus  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanu","family":"Bhati","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Laboratory ICMR\u2010National Institute of Pathology, Sriramachari Bhawan, Safdarjung Hospital Campus  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astha","family":"Dimri","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Laboratory ICMR\u2010National Institute of Pathology, Sriramachari Bhawan, Safdarjung Hospital Campus  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Vardhman Mahavir Medical College (VMMC) and Safdarjung Hospital  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fouzia","family":"Siraj","sequence":"additional","affiliation":[{"name":"Pathology Laboratory ICMR\u2010National Institute of Pathology, Sriramachari Bhawan, Safdarjung Hospital Campus  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0402-909X","authenticated-orcid":false,"given":"Sangita","family":"Rastogi","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Laboratory ICMR\u2010National Institute of Pathology, Sriramachari Bhawan, Safdarjung Hospital Campus  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.03.019"},{"issue":"10","key":"e_1_2_11_3_1","first-page":"QC13","article-title":"Clinical analysis of ectopic pregnancies in a tertiary care centre in Southern India: a six\u2010year retrospective study","volume":"10","author":"Tahmina S","year":"2016","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2010.11.019"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02564.x"},{"issue":"6","key":"e_1_2_11_6_1","first-page":"591","article-title":"Chlamydia infection as a risk factor in ectopic pregnancy: a case control study","volume":"13","author":"Chandramathi P","year":"2021","journal-title":"Int J Reprod Contracept Obstet Gynecol"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2005.02840.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jog.13251"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2002.126850"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00283"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2018.05.033"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060175"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12041-016-0675-5"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1371425"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2009.00536.x"},{"issue":"3","key":"e_1_2_11_16_1","first-page":"88","article-title":"Evaluation of diagnostic efficacy of PCR methods for Chlamydia trachomatis infection in genital and urine specimens of symptomatic men and women in India","volume":"56","author":"George JA","year":"2003","journal-title":"Jpn J Infect Dis"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.06.031"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-7-40"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.5603\/GP.a2021.0089"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/15513815.2019.1710879"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2022.105468"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2013.1"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2022.02.019"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-017-0939-x"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01277"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13260"}],"container-title":["International Journal of Gynecology &amp; Obstetrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijgo.15547","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T07:05:03Z","timestamp":1719126303000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/ijgo.15547"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,22]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/ijgo.15547"],"URL":"https:\/\/doi.org\/10.1002\/ijgo.15547","archive":["Portico"],"relation":{},"ISSN":["0020-7292","1879-3479"],"issn-type":[{"value":"0020-7292","type":"print"},{"value":"1879-3479","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,22]]},"assertion":[{"value":"2023-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}