{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T10:37:12Z","timestamp":1788259032457,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J Gynecology &amp; Obste"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to determine whether circulating tumor human papillomavirus (HPV) DNA is a potential specific biomarker for cervical cancer (CC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This retrospective matched study included 87 patients with cervical intraepithelial neoplasia (CIN), 29 CC patients (FIGO IA1\u2010IVA) and 29 HPV\u2010negative controls at Yuhuangding Hospital of Qingdao University (from July 2022 to September 2023). The digital droplet PCR (ddPCR) was used to detect and quantify ctHPV DNA in the plasma of patients with HPV16, 18, 33, 52, or 58\u2010associated CC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The ctHPV DNA was exclusively detectable in HPV\u2010positive samples, with no detection in patients across various CIN stages (<jats:italic>n<\/jats:italic>\u2009=\u200987) or HPV\u2010negative controls (<jats:italic>n<\/jats:italic>\u2009=\u200929). Additionally, ctHPV DNA was identified in nine out of 10 late\u2010stage patients (90%) and six out of 19 early\u2010stage patients (31.6%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The ctHPV DNA serves as a specific biomarker for the diagnosis CC. Additionally, this discovery addresses the knowledge gap in ctHPV DNA research in the early stages of CC and promotes clinical diagnosis and treatment strategies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ijgo.16011","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T13:45:21Z","timestamp":1731073521000},"page":"148-154","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Circulating tumor HPV DNA as a specific biomarker for cervical cancer"],"prefix":"10.1002","volume":"169","author":[{"given":"Tao","family":"Chen","sequence":"first","affiliation":[{"name":"Guangzhou KingMed Transformative Medicine Institute Co. Ltd. and Guangzhou KingMed Diagnostics Group Co. Ltd.  Guangzhou China"},{"name":"KingMed School of Laboratory Medicine Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology Guangzhou KingMed Center for Clinical Laboratory  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumei","family":"Sun","sequence":"additional","affiliation":[{"name":"Guangzhou KingMed Transformative Medicine Institute Co. Ltd. and Guangzhou KingMed Diagnostics Group Co. Ltd.  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Yantai Yuhuangding Hospital of Qingdao University  Yantai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanran","family":"Tao","sequence":"additional","affiliation":[{"name":"KingMed School of Laboratory Medicine Guangzhou Medical University  Guangzhou China"},{"name":"Department of Pathology Guangzhou KingMed Center for Clinical Laboratory  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juming","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pathology Guangzhou KingMed Center for Clinical Laboratory  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujun","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Affiliated Yantai Yuhuangding Hospital of Qingdao University  Yantai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(00)00376-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28149"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3066"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-21-0625"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.34333"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11142170"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2019.03.005"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01480-21"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-17-1553"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370220978899"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2022.07.028"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-023-05077-3"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006250-200312000-00022"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cjp2.47"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188592"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-21-3151"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2014.08.001"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2020.08.002"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.23.00954"}],"container-title":["International Journal of Gynecology &amp; Obstetrics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijgo.16011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T08:35:37Z","timestamp":1743582937000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/ijgo.16011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,8]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/ijgo.16011"],"URL":"https:\/\/doi.org\/10.1002\/ijgo.16011","archive":["Portico"],"relation":{},"ISSN":["0020-7292","1879-3479"],"issn-type":[{"value":"0020-7292","type":"print"},{"value":"1879-3479","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,8]]},"assertion":[{"value":"2024-06-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}