{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T08:18:58Z","timestamp":1771316338135,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J Gynecology &amp; Obste"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To explore the relationships between HPV genotypes, vaginal microbiota, oncogenic mutations, and cervical intraepithelial neoplasia (CIN) to develop a risk assessment model for predicting CIN.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A cross\u2010sectional analysis was conducted on 264 women living in the Kaliningrad region, assessing CIN presence, HPV genotypes, vaginal microbiota composition, and mutations in key oncogenes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HPV genotypes 16, 31, 33, 35, 58, and 66 were identified as the most prevalent among patients with HPV infections. However, in a multifactorial model, only HPV genotypes 16 and 58 demonstrated a significant association with high\u2010grade squamous intraepithelial lesions and above. Genetic analysis revealed mutations in\n                      <jats:italic>TP53<\/jats:italic>\n                      and\n                      <jats:italic>ERBB2<\/jats:italic>\n                      genes in 20 and 17 patients, respectively, with\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations showing a notable correlation with CIN progression. Two patients with diagnoses of \u201cNegative for Intraepithelial Lesion or Malignancy\u201d carried the\n                      <jats:italic>TP53<\/jats:italic>\n                      R248W mutation, a well\u2010established neoplasia\u2010related variant, highlighting its potential as a predictor of precancerous conditions. Increased copy numbers of human DNA and\n                      <jats:italic>Enterobacteriaceae<\/jats:italic>\n                      DNA correlated with low\u2010grade squamous intraepithelial lesions, though many DNA\u2010derived features displayed instability in logistic regression models, suggesting the need for further validation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings suggest that although HPV genotypes, genetic mutations, and microbiota profiles may serve as markers for CIN, their predictive reliability requires further investigation. The present study represents the first large\u2010scale exploration of these factors conducted within the Russian female population.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ijgo.70090","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T11:44:08Z","timestamp":1746445448000},"page":"916-926","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive cross\u2010sectional study of\n                    <scp>CIN<\/scp>\n                    prevalence,\n                    <scp>HPV<\/scp>\n                    genotyping, genetic alterations, and microbiota as molecular biomarkers for early cervical cancer detection: A pilot clinical study among women in Russia"],"prefix":"10.1002","volume":"170","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0583-443X","authenticated-orcid":false,"given":"Alexander","family":"Romanishin","sequence":"first","affiliation":[{"name":"Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University  Kaliningrad Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7635-3329","authenticated-orcid":false,"given":"Bogdan","family":"Efimenko","sequence":"additional","affiliation":[{"name":"Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University  Kaliningrad Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6780-1820","authenticated-orcid":false,"given":"Dmitriy","family":"Katserov","sequence":"additional","affiliation":[{"name":"Institute of Medicine and Life Sciences, Immanuel Kant Baltic Federal University  Kaliningrad 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