{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:09:42Z","timestamp":1791220182233,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intl J Gynecology &amp;amp; Obste"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To explore the role of a DNA methylation assay for managing minimally abnormal cervical cancer screening results in a prospective cohort undergoing opportunistic cervical cancer screening.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In the cohort of the METHY2 and METHY3 screening studies of women undergoing opportunistic cervical cancer screening, cervical cytology samples were sent for high\u2010risk human papillomavirus (hrHPV) DNA assays, cytologic pathology and methylation assays of\n                      <jats:italic>PAX1<\/jats:italic>\n                      \/\n                      <jats:italic>JAM3<\/jats:italic>\n                      (CISCER). This study evaluated the discriminative power of CISCER in managing women with minimally abnormal cervical cancer screening results for CIN3+. Absolute CIN3+ risks and colposcopy referrals within one screening round were calculated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 1857 women with minimally abnormal cervical cancer findings had cervical histologic outcomes and were included in the analysis. In women with a minimally abnormal cervical cancer result, the sensitivity and specificity of CISCER was 74.9% (95% confidence interval [CI], 68.3%\u201381.4%) and 89.1% (95% CI 87.6%\u201390.6%) for detecting CIN3+. CISCER analysis discriminated well for minimally abnormal cervical cancer results, yielding a CIN3+ risk of 40.5% (95% CI 34.9%\u201346.2%) after a positive result and a CIN3+ risk of 2.7% (95% CI 2.0%\u20133.6%) after a negative result.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In women with a minimally abnormal cervical cancer screening result, the CISCER provides excellent detection of CIN3+. The use of CISCER in women with a minimally abnormal cervical cancer screening result can lead to a substantial reduction in the number of direct colposcopy referrals.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ijgo.70167","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T11:28:36Z","timestamp":1744802916000},"page":"405-414","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Cytologic\n                    <scp>DNA<\/scp>\n                    methylation for managing minimally abnormal cervical cancer screening results"],"prefix":"10.1002","volume":"171","author":[{"given":"Xiao","family":"Shang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking Union Medical College Hospital  Beijing China"},{"name":"National Clinical Research Center for Obstetric &amp; Gynecologic Diseases  Beijing China"},{"name":"State Key Laboratory for Complex, Severe and Rare Diseases Peking Union Medical College Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linghua","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking Union Medical College Hospital  Beijing China"},{"name":"National Clinical Research Center for Obstetric &amp; Gynecologic Diseases  Beijing China"},{"name":"State Key Laboratory for Complex, Severe and Rare Diseases Peking Union Medical College Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"You","sequence":"additional","affiliation":[{"name":"Department of Pathology Peking Union Medical College Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanwen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology Peking Union Medical College Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuligh","family":"Liou","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Beijing Origin\u2010Poly Bio\u2010Tec Co., Ltd.  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