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Emerging evidence suggests that reproductive disorders may be closely associated with intestinal damage or occasionally worsen by the side effects of medications. Therefore, the development of dietary supplements as alternatives is crucial for intestinal\u2010linked reproductive health. Milk and dairy products are essential in dietary nutrition with great potential functional ingredients.\n                    <jats:italic>Bifidobacterium animalis<\/jats:italic>\n                    subsp\n                    <jats:italic>. lactis<\/jats:italic>\n                    NJ241 (NJ241), a promising probiotic isolated from naturally fermented bovine milk, remains underexplored. This study aims to investigate the molecular mechanisms of NJ241 on colitis and its associated reproductive disorders. The biomarker microbes and their correlated metabolites were further explored by 16S rRNA sequencing and metabolomics. Hematological analysis and histopathological examination were applied for conjoined identification. Results indicated that NJ241 effectively restored the expression levels of Claudin\u20102 and MLCK1, reducing intestinal permeability. Multi\u2010omics results further revealed that NJ241 may effectively improve gut barrier integrity by increasing the abundance of\n                    <jats:italic>Akkermansia muciniphila<\/jats:italic>\n                    and its metabolite trans\u2010ferulic acid. This effect was accompanied by a reduction in the pro\u2010inflammatory cytokine IL\u20106 in both serum and testicular tissue, mediated through the TLR4 signaling pathway. Consequently, the restoration of microbiota homeostasis and a systemic reduction in inflammation rescued testicular spermatogenesis, which was impaired by colitis. The current findings consistently elucidated the potential molecular mechanism by which NJ241 ameliorates colitis\u2010linked reproductive disorders through the gut\u2010testis axis. Additionally, NJ241 demonstrates promise as a probiotic supplement for the development of fortified dairy products and provides strong evidence for the potential reproductive health benefits of naturally fermented bovine milk.\n                  <\/jats:p>","DOI":"10.1002\/imo2.49","type":"journal-article","created":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T01:09:12Z","timestamp":1735866552000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dietary supplementation with milk\u2010derived\n                    <i>Bifidobacterium animalis<\/i>\n                    subsp\n                    <i>. lactis<\/i>\n                    relieves colitis\u2010linked reproductive disorders via gut\u2010testis axis"],"prefix":"10.1002","volume":"2","author":[{"given":"Jingmin","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Food Science and Engineering, College of Life Science and Technology Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingzi","family":"Yin","sequence":"additional","affiliation":[{"name":"Bioscience and Biomedical Engineering Thrust, Systems Hub The Hong Kong University of Science and Technology (Guangzhou) Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyao","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Food Science and Engineering, College of Life Science and Technology Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiling","family":"Yu","sequence":"additional","affiliation":[{"name":"Centre for Cancer and Inflammation Research, School of Chinese Medicine Hong Kong Baptist University Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuqiong","family":"Fu","sequence":"additional","affiliation":[{"name":"Centre for Cancer and Inflammation Research, School of Chinese Medicine Hong Kong Baptist University Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institutes for Systems Genetics, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaojun","family":"Tang","sequence":"additional","affiliation":[{"name":"Bioscience and Biomedical Engineering Thrust, Systems Hub The Hong Kong University of Science and Technology (Guangzhou) Guangzhou China"},{"name":"Center for Aging Science, Division of Emerging Interdisciplinary Areas The Hong Kong University of Science and Technology Hong Kong 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