{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T12:21:49Z","timestamp":1765628509136,"version":"3.48.0"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T00:00:00Z","timestamp":1759622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["iMetaOmics"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The adipose tissue (AT) is a main regulator of systemic energy homeostasis, and AT \ufeffdysfunction leads to insulin resistance and other metabolic complications. Protein arginine methyltransferase 5 (PRMT5) catalyzes symmetrical dimethylation of arginine residues to modulate protein stability and function. Besides its well\u2010studied oncogenic functions, PRMT5 has recently been shown to play a physiological role in AT through poorly understood mechanisms. Here, we explore the function of PRMT5 in AT through unbiased RNA sequencing and lipidomic analyses of AT in wild\u2010type and adipocyte\u2010specific\n                    <jats:italic>Prmt5<\/jats:italic>\n                    knockout (\n                    <jats:italic>Prmt5<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>AKO<\/jats:italic>\n                    <\/jats:sup>\n                    ) mice. Transcriptomic profiling revealed that\n                    <jats:italic>Prmt5<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>AKO<\/jats:italic>\n                    <\/jats:sup>\n                    alters the expression of genes related to metabolism and membrane transport. Specifically,\n                    <jats:italic>Prmt5<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>AKO<\/jats:italic>\n                    <\/jats:sup>\n                    induces genes enriched in glucose transport and glycolysis pathways, while suppressing genes encoding fatty acid (FA) transporters. Lipidomics analysis showed altered compositions of triacylglycerols (TAGs), fatty acids (FAs), and glycerophospholipids. Additionally,\n                    <jats:italic>Prmt5<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>AKO<\/jats:italic>\n                    <\/jats:sup>\n                    promotes cholesterol biogenesis, associated with hyperlipidemia and hepatic steatosis in mice. These multiomics approaches uncover previously unappreciated roles of PRMT5 as an epigenetic regulator of metabolic homeostasis via coordinating membrane transport, balancing glucose and FA metabolism, and promoting cholesterol biosynthesis. This study highlights a novel mechanism by which protein methylation regulates systemic energy balance.\n                  <\/jats:p>","DOI":"10.1002\/imo2.70055","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T03:59:37Z","timestamp":1759723177000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multiomics analyses reveal that PRMT5 regulates membrane transport and cholesterol synthesis in white adipocytes"],"prefix":"10.1002","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0598-6189","authenticated-orcid":false,"given":"Xiyue","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Animal Sciences Purdue University West Lafayette Indiana 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