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This study investigated the gut\u2010mammary gland axis and how the prebiotic candidate ginseng polysaccharide (GP) prevents mastitis. Using Lipopolysaccharide (LPS)\u2010induced mouse models, we demonstrate that mastitis involves gut dysbiosis contributing to pathogenesis. GP prevented mastitis in a microbiota\u2010dependent manner. Mechanistically, GP selectively enriches\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    (\n                    <jats:italic>L<\/jats:italic>\n                    .)\n                    <jats:italic>murinus<\/jats:italic>\n                    , boosting its metabolite deoxycholic acid (DCA) level in the bloodstream and mammary tissue. DCA suppressed inflammation by binding TGR5 (Takeda G\u2010protein\u2010coupled receptor 5), activating the cAMP\u2010PKA (cyclic adenosine monophosphate\u2010protein kinase A) pathway, and inhibiting NF\u2010\u03baB\/NLRP3 inflammasome. Additionally, combining GP with\n                    <jats:italic>L. murinus<\/jats:italic>\n                    enhanced protection, suggesting potential as a synbiotic. Overall, these findings reveal a novel gut\u2010mammary gland axis where GP acts by enhancing\n                    <jats:italic>L. murinus<\/jats:italic>\n                    \u2010derived DCA and TGR5 signaling, highlighting the therapeutic potential of targeting this axis for mastitis prevention and management.\n                  <\/jats:p>","DOI":"10.1002\/imo2.70090","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:01:02Z","timestamp":1772989262000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ginseng polysaccharides prevent mastitis through\n                    <i>Lactobacillus murinus<\/i>\n                    \u2010derived deoxycholic acid and TGR5 signaling"],"prefix":"10.1002","volume":"3","author":[{"given":"Zhijie","family":"Zheng","sequence":"first","affiliation":[{"name":"MOA Key Laboratory of Animal Virology Zhejiang University Center for Veterinary Sciences 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