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While the gut microbiota has been implicated in PCOS pathophysiology, the role of microbial\u2010derived metabolites as mediators of host\u2013microbe interactions remains poorly defined. Here, we integrated untargeted gut metabolomics with metagenomic profiling in patients with PCOS and identified a marked depletion of 3,4\u2010dihydroxyphenylacetic acid (DHPAA), a flavonoid\u2010derived microbial catabolite. Oral administration of DHPAA ameliorated PCOS\u2010like phenotypes in two mouse models by suppressing bone morphogenetic protein signaling and reducing anti\u2010M\u00fcllerian hormone (AMH) levels. We found that DHPAA production depends on gut microbial degradation of dietary flavonoids. We further identified a bacterial species, <jats:italic>Streptococcus thermophilus<\/jats:italic>, consistently depleted in PCOS across two human cohorts and a mouse model, restored DHPAA levels and improved reproductive outcomes in mice. Conversely, a \u03b2\u2010galactosidase\u2010deficient mutant of <jats:italic>S. thermophilus<\/jats:italic> failed to confer these benefits, highlighting \u03b2\u2010galactosidase as a critical enzyme in DHPAA biosynthesis. 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