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Since its initial release in late 2020, the R microeco package has been widely used for downstream statistical analysis and visualization of omics data, such as amplicon sequencing. Compared with its initial release, the current second version of the microeco package has undergone extensive updates and enhancements. The key upgrades include: (1) The addition of classes for data normalization and machine learning, respectively; (2) The incorporation of additional analytical methods and the addition of functions across various classes; (3) Optimization of the parameter system to expand the applicable scenarios of relevant methods; (4) Code restructuring to enhance the connectivity between statistical analysis and visualization within each class; (5) Extension of certain functions to enable the analysis of abundance data in complex formats generated from bioinformatic analyses of metagenomic\/metatranscriptomic data; (6) Incorporation of several analytical methods commonly used in transcriptomic and metabolomic data analyses. Overall, the microeco package 2.0 offers broader method coverage and a wider range of application scenarios compared to the previous version and other existing R packages. The steady growth in user downloads demonstrates that the microeco package, which is built on R6 (a class\u2010based object\u2010oriented programming system for R), has established a broad and active user base. The second version of the microeco R package is open\u2010source and available on the Comprehensive R Archive Network and GitHub (\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/github.com\/ChiLiubio\/microeco\">https:\/\/github.com\/ChiLiubio\/microeco<\/jats:ext-link>\n                    ).\n                  <\/jats:p>","DOI":"10.1002\/imt2.70132","type":"journal-article","created":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T09:37:54Z","timestamp":1778924274000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["microeco 2: A comprehensive R package for downstream analysis of microbiome omics data"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4055-8677","authenticated-orcid":false,"given":"Chi","family":"Liu","sequence":"first","affiliation":[{"name":"Engineering Research Center of Soil Remediation of Fujian Province University; College of Resources and Environment, Fujian Agriculture and Forestry University Fuzhou China"},{"name":"State Key Laboratory of High\u2010Efficiency Production of Wheat\u2010Maize Double Cropping\/College of Agronomy Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangzhen","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Soil Remediation of Fujian Province University; College of Resources and Environment, Fujian Agriculture and Forestry University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe R. 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