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Although brain\u2010derived EVs (bdEVs) have been successfully collected from brain tissue, there is not yet a \u201cbdEV Atlas\u201d of EVs from different brain regions. To address this gap, we separated EVs from eight anatomical brain regions of a single individual and subsequently characterized them by count, size, morphology, and protein and RNA content. The greatest particle yield was from cerebellum, while the fewest particles were recovered from the orbitofrontal, postcentral gyrus, and thalamus regions. EV surface phenotyping indicated that CD81 and CD9 were more abundant than CD63 in all regions. Cell\u2010enriched surface markers varied between brain regions. For example, putative neuronal markers NCAM, CD271, and NRCAM were more abundant in medulla, cerebellum, and occipital regions, respectively. These findings, while restricted to tissues from a single individual, suggest that additional studies are warranted to provide more insight into the links between EV heterogeneity and function in the CNS.<\/jats:p>","DOI":"10.1002\/inmd.20230016","type":"journal-article","created":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T12:11:28Z","timestamp":1692187888000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Toward a human brain extracellular vesicle atlas: Characteristics of extracellular vesicles from different brain regions, including small RNA and protein profiles"],"prefix":"10.1002","volume":"1","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1749-963X","authenticated-orcid":false,"given":"Yiyao","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Molecular and Comparative Pathobiology Johns Hopkins University School of Medicine  Baltimore Maryland 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