{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:28:06Z","timestamp":1791412086914,"version":"4.3.3"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,11,7]],"date-time":"2014-11-07T00:00:00Z","timestamp":1415318400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aim of this study is to investigate the antifungal mechanism of curcumin. This polyphenolic compound has been used traditionally in Asia for medicinal, culinary, and other purposes. Although antifungal effect of curcumin has been reported, this is the first study for its mode of action underlying disruption of plasma membrane in\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    . The leakage of potassium ion from the fungal cytosol and dissipation in membrane potential was detected by bis\u2010(1,3\u2010dibutylbarbituric acid)trimethine oxonol [DiBAC\n                    <jats:sub>4<\/jats:sub>\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#iub1326-bib-0003\"\/>\n                    ] staining. We also investigated an increase in membrane permeability in curcumin\u2010treated\n                    <jats:italic>C. albicans<\/jats:italic>\n                    with influx of propidium iodide assay. Fluorescence analysis with 1,6\u2010diphenyl\u20101,3,5\u2010hexatriene supported the membrane\u2010targeted mechanism of action indicating membrane disruption. On the basis of these results, we studied the effects of curcumin treatment on model membrane to elucidate its antifungal mechanism. Using calcein leakage assays from curcumin\u2010treated large unilamellar vesicles and giant unilamellar vesicles, we found that curcumin has membrane\u2010active mechanism inducing leakage of intracellular component through the flappy membrane. Therefore, this study suggests that curcumin exerts antifungal activity via inducing disruption of fungal plasma membrane. \u00a9 2014 IUBMB Life, 66(11):780\u2013785, 2014\n                  <\/jats:p>","DOI":"10.1002\/iub.1326","type":"journal-article","created":{"date-parts":[[2014,11,7]],"date-time":"2014-11-07T07:57:57Z","timestamp":1415347077000},"page":"780-785","source":"Crossref","is-referenced-by-count":110,"title":["An antifungal mechanism of curcumin lies in membrane\u2010targeted action within\n                    <scp>\n                      <i>C<\/i>\n                    <\/scp>\n                    <i>andida albicans<\/i>"],"prefix":"10.1002","volume":"66","author":[{"given":"Wonyoung","family":"Lee","sequence":"first","affiliation":[{"name":"School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University Buk\u2010Gu Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Gun","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University Buk\u2010Gu Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/b914961a"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/344658"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1709.101987"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00705-08"},{"key":"e_1_2_5_6_1","first-page":"4781","article-title":"Analysis of the tumoricidal and anti\u2010cachectic potential of curcumin","volume":"34","author":"Oelkrug C.","year":"2014","journal-title":"Anticancer Res."},{"key":"e_1_2_5_7_1","first-page":"9384","article-title":"Therapeutic applications of curcumin for patients with pancreatic cancer","volume":"20","author":"Kanai M.","year":"2014","journal-title":"World J. 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