{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T09:23:56Z","timestamp":1768814636827,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,3]],"date-time":"2015-04-03T00:00:00Z","timestamp":1428019200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81270514"],"award-info":[{"award-number":["81270514"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30873424"],"award-info":[{"award-number":["30873424"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Doctoral Discipline Foundation of Ministry of Education of China","award":["20103237110010"],"award-info":[{"award-number":["20103237110010"]}]},{"name":"National Science and Technology Supporting Program","award":["2008BAI51B02"],"award-info":[{"award-number":["2008BAI51B02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatic fibrosis is concomitant with liver inflammation, which has been highlighted as significant treatment of chronic liver disease. We previously demonstrated that tetramethylpyrazine (TMP), the effective component of Ligusticum chuanxiong Hort, can inhibit the activation of HSCs and consequential anti\u2010hepatic fibrosis. In this study, our work demonstrated that TMP improved liver histological architecture, decreased hepatic enzyme levels and attenuated collagen deposition in the rat fibrotic liver. In addition, TMP significantly protected the liver from CCl4\u2010caused injury and fibrogenesis by suppressing inflammation with reducing levels of inflammatory cytokines, including tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), NLRP3, <jats:bold>nuclear factor\u2010kappa<\/jats:bold> B (NF\u2010\u03baB) and interleukin\u20101\u03b2 (IL\u20101\u03b2). Experiments <jats:italic>in vitro<\/jats:italic> showed that TMP inhibited inflammatory cytokine expression in HSCs associated with disrupting platelet\u2010derived growth factor\u2010b receptor (PDGF\u2010\u03b2R)\/NLRP3\/caspase1 pathway. These data collectively indicate that TMP can attenuate liver inflammation in liver fibrosis and possibly by targeting HSCs via PDGF\u2010\u03b2R\/NLRP3\/caspase1 pathway. It provides novel mechanistic insights into TMP as a potential therapeutic remedy for hepatic fibrosis. \u00a9 2015 IUBMB Life, 67(4):312\u2013321, 2015<\/jats:p>","DOI":"10.1002\/iub.1348","type":"journal-article","created":{"date-parts":[[2015,4,3]],"date-time":"2015-04-03T18:49:18Z","timestamp":1428086958000},"page":"312-321","source":"Crossref","is-referenced-by-count":82,"title":["Tetramethylpyrazine reduces inflammation in liver fibrosis and inhibits inflammatory cytokine expression in hepatic stellate cells by modulating <scp>NLRP<\/scp>3 inflammasome pathway"],"prefix":"10.1002","volume":"67","author":[{"given":"Xiafei","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Nanjing University of Chinese Medicine Nanjing China"},{"name":"National First\u2010Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine Nanjing China"},{"name":"Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Material Medical Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunfeng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naqi","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Nanjing University of Chinese Medicine Nanjing China"},{"name":"National First\u2010Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine Nanjing China"},{"name":"Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Material Medical Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shizhong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, College of Pharmacy Nanjing University of Chinese Medicine Nanjing China"},{"name":"National First\u2010Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine Nanjing China"},{"name":"Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Material Medical Nanjing University of Chinese Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(02)00429-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20235"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10809"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v17.i43.4772"},{"key":"e_1_2_6_6_1","first-page":"305","article-title":"Acetaminophen\u2010induced hepatotoxicity in mice is dependent on Tlr9 and the Nalp3 inflammasome","volume":"119","author":"Imaeda A. B.","year":"2009","journal-title":"J. Clin. Invest."},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510310117"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2741\/A875"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2010.97"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2004.03.006"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.128306"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i10.2515"},{"key":"e_1_2_6_13_1","first-page":"531","article-title":"Effect of ligustrazine hydrochloride on coagulation reaction and inflammation reaction in single valve replacement patients with rheumatic heart disease undergoing cardiopulmonary bypass","volume":"34","author":"Chen Y. J.","year":"2014","journal-title":"Chin. J. Integr. Tradit.West. Med."},{"key":"e_1_2_6_14_1","first-page":"868","article-title":"Effect of ligustrazine on expression of RhoA mRNA, ROCK\u2010II protein in the lung and airway inflammation of allergic asthma model mice","volume":"46","author":"Luo Y. C.","year":"2008","journal-title":"Chin. J. Pediatrics"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12818"},{"key":"e_1_2_6_16_1","first-page":"153","article-title":"Effect of ligustrazine on the expression of TNF\u2010a, IL\u20104 and IFN\u2010\u03b3 in rats with liver fibrosis","volume":"31","author":"Chen W.","year":"2010","journal-title":"World Clin. Drug."},{"key":"e_1_2_6_17_1","first-page":"555","article-title":"The role of thrombocytes in liver fibrogenesis: effects of platelet lysate and thrombocyte\u2010derived growth factors on the mitogenic activity and glycosaminoglycan synthesis of cultured rat liver fat storing cells","volume":"27","author":"Bachem M. G.","year":"1989","journal-title":"J. Clin. Chem. Clin. Biochem."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.2741\/A812"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.2741\/A887"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12286"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/157488810791268636"},{"key":"e_1_2_6_22_1","first-page":"438","article-title":"Hepatic stellate cells (HSC): architects of hepatic fibrosis","volume":"95","author":"Solis\u2010Herruzo J. A.","year":"2003","journal-title":"Rev. Esp. Enferm. Dig."},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(03)00157-4"},{"key":"e_1_2_6_24_1","first-page":"785","article-title":"Expression of platelet\u2010derived growth factor and its receptors in normal human liver and during active hepatic fibrogenesis","volume":"148","author":"Pinzani M.","year":"1996","journal-title":"Am. J. Pathol."},{"key":"e_1_2_6_25_1","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S1665-2681(19)30918-4","article-title":"Chemokines: potent mediators of hepatic inflammation and fibrosis in chronic liver diseases","volume":"13","author":"Handa P.","year":"2013","journal-title":"Ann. Hepatol."},{"key":"e_1_2_6_26_1","first-page":"1996","article-title":"Relationship of oxygen and glutathione in protection against carbon tetrachloride\u2010induced hepatic microsomal lipid peroxidation and covalent binding in the rat","volume":"74","author":"Burk R. F.","year":"1984","journal-title":"Rationale for the use of hyperbaric oxygen to treat carbon tetrachloride ingestion. J. Clin. Invest."},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003330"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00599-3"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90223.2008"}],"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fiub.1348","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fiub.1348","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.1348","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T09:55:36Z","timestamp":1696326936000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/iub.1348"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1002\/iub.1348"],"URL":"https:\/\/doi.org\/10.1002\/iub.1348","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4]]}}}