{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:10:54Z","timestamp":1761217854272},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,3,7]],"date-time":"2016-03-07T00:00:00Z","timestamp":1457308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stem cell therapy is considered as a promising approach in the treatment of myocardial infarction (MI). This study was designed as a comparison of human umbilical cord blood (HUCB)\u2010derived CD34+ and HUCB\u2010derived MSCs for the repair of cardiac tissue by induction of the angiogenesis. Forty\u2010eight male rats were randomized into four groups: sham\u2010operated group, MI group, MSCs\u2010treated group, and CD34+ cells\u2010treated group. After 4 weeks, the rats were sacrificed. All sections from left ventricles of all groups were subjected to hematoxylin &amp; eosin, Masson's trichrome, and immunohistochemical stains (CD133, CD44, and \u03b1\u2010smooth muscle actin). RNA was extracted for gene expression of the angiogenic markers. A significant reduction of the infarct size and the amplitude of T\u2010wave in the CD34+ cells\u2010treated group when compared with the MSCs\u2010treated group were determined. Histologically, the MI group showed scar tissue, congested blood capillaries around the infarcted area, some necrotic cells, and inflammatory cells. Administration of either MSCs or CD34+ cells had a therapeutic potential to induce regenerative changes in the myocardium with better results in CD34+cells\u2010treated group. Quantitative RT\u2010PCR analysis revealed a significant increase in the expression of vascular endothelial growth factor (VEGF), VEGFR\u20102, Ang\u20101, and Tie\u20102 and a significant decreased expression of Ang\u20102 in stem cells transplanted groups when compared with the noncell transplanted hearts. A significant increase of VEGF, VEGFR\u20102, Ang\u20101, and Tie\u20102 expression in the group receiving CD34+ cells than those receiving MSCs was found. Finally, there was an upregulation of both human VEGF and human hypoxia\u2010inducible factor 1\u03b1 in the infarcted hearts treated by CD34+ cells than that treated by MSCs. We first revealed a superior efficacy of CD34+ cells when compared with MSCs in induction of regenerative changes in the MI model. Both cell therapies may repair the damaged heart tissue primarily by secretion of proangiogenic factors that induce the angiogenesis and activate the angiogenesis signaling pathway. \u00a9 2016 IUBMB Life, 68(5):343\u2013354, 2016<\/jats:p>","DOI":"10.1002\/iub.1487","type":"journal-article","created":{"date-parts":[[2016,3,7]],"date-time":"2016-03-07T13:01:33Z","timestamp":1457355693000},"page":"343-354","source":"Crossref","is-referenced-by-count":11,"title":["Comparing the effects of MSCs and CD34+ cell therapy in a rat model of myocardial infarction"],"prefix":"10.1002","volume":"68","author":[{"given":"Sally M.","family":"Shalaby","sequence":"first","affiliation":[{"name":"Medical Biochemistry Department Faculty of Medicine, Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amal S.","family":"El\u2010Shal","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department Faculty of Medicine, Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haidy E.","family":"Zidan","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department Faculty of Medicine, Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nehad F.","family":"Mazen","sequence":"additional","affiliation":[{"name":"Histology and Cell Biology Department Faculty of Medicine, Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manal R.","family":"Abd El\u2010Haleem","sequence":"additional","affiliation":[{"name":"Histology and Cell Biology Department Faculty of Medicine, Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalia M.","family":"Abd El Motteleb","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology Department Faculty of Medicine, Zagazig University Zagazig Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1112570"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-011-9240-2"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.02.005"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-011-9577-x"},{"key":"e_1_2_5_6_1","first-page":"261","article-title":"Umbilical cord blood: current status & promise for the future","volume":"134","author":"McKenna D.","year":"2011","journal-title":"Indian J. 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