{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T02:42:19Z","timestamp":1767840139719,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T00:00:00Z","timestamp":1462752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>PorB is a pan\u2010Neisserial major outer membrane protein with a trimeric \u03b2\u2010barrel structure. Each monomer presents eight periplasmic turns and eight surface exposed loop regions with sequence variability. PorB induces activation of host cell responses via a TLR2\u2010dependent mechanism likely mediated by electrostatic interactions between TLR2 and PorB surface exposed loops. Variability in the loop amino acid sequence is known to influence cell responses to PorB <jats:italic>in vitro<\/jats:italic>, particularly for the residues in L5 and L7. In this work, the sequence of the <jats:italic>porB<\/jats:italic> gene and the electrostatic surface charges of PorB from 35 invasive meningococcal isolates belonging to the main clonal complexes identified in Italy and from five carriage genomes available on the website <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/pubmlst.org\/neisseria\/\">http:\/\/pubmlst.org\/neisseria\/<\/jats:ext-link> were examined. Analysis of the <jats:italic>porB<\/jats:italic> encoding regions from the invasive meningococci has identified four new alleles and a potential association between <jats:italic>porB<\/jats:italic> alleles, serogroup, and clonal complexes. Through computer\u2010based modeling and analysis of the electrostatic surface charges of PorB from these strains, loop charge segregation between PorB from invasive serogroups B and C was observed. Specifically, loops 1, 4, and 7 were negatively charged and L2 and L8 were mostly neutral in serogroup B isolates, while an overall homogeneous positive surface charge was present in PorB from invasive serogroup C strains. A higher PorB sequence variability was observed among carriage genomes, and a general prevalence of negative loop surface charges. The surface charge differences in PorB from serogroups B and C invasive and carriage strains may, in part, influence the outcomes of <jats:italic>Neisseriae<\/jats:italic> interactions with host cells. \u00a9 2016 IUBMB Life, 68(6):488\u2013495, 2016<\/jats:p>","DOI":"10.1002\/iub.1508","type":"journal-article","created":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T05:12:46Z","timestamp":1462770766000},"page":"488-495","source":"Crossref","is-referenced-by-count":7,"title":["Typing and surface charges of the variable loop regions of PorB from <i>Neisseria meningitidis<\/i>"],"prefix":"10.1002","volume":"68","author":[{"given":"Paola","family":"Stefanelli","sequence":"first","affiliation":[{"name":"Department of Infectious, Parasitic &amp; Immuno\u2010Mediated Diseases Istituto Superiore Di Sanit\u00e0 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arianna","family":"Neri","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic &amp; Immuno\u2010Mediated Diseases Istituto Superiore Di Sanit\u00e0 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikio","family":"Tanabe","sequence":"additional","affiliation":[{"name":"Structural Biology Research Center, Photon Factory Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK) Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Fazio","sequence":"additional","affiliation":[{"name":"Department of Infectious, Parasitic &amp; Immuno\u2010Mediated Diseases Istituto Superiore Di Sanit\u00e0 Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Massari","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology and Pathobiology Tufts University School of Medicine Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.04.070"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20090513"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2009.12.004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2014.11.006"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.11.002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00215-06"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.5.941"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi000241j"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/322784"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3416-3419.1998"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01230.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00057.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912115107"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2013.12.006"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20121025"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-014-1473-2"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01487.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2406-2413.1999"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)00037-9"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.10.41"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046233"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.4.671"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.7.4489"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.4.1533"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.4.2373"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00681-10"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00683-12"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-135"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139376"},{"key":"e_1_2_6_31_1","doi-asserted-by":"crossref","first-page":"3680","DOI":"10.1128\/JCM.36.12.3680-3682.1998","article-title":"Heterogeneity of the PorB protein in serotype 22 Neisseria meningitidis","volume":"36","author":"Urwin R.","year":"1998","journal-title":"J. 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