{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T19:10:02Z","timestamp":1749928202821,"version":"3.41.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,1,23]],"date-time":"2017-01-23T00:00:00Z","timestamp":1485129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003711","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 105-2320-B-075A-002","NSC 102-2314-B-075A-011-MY2"],"award-info":[{"award-number":["MOST 105-2320-B-075A-002","NSC 102-2314-B-075A-011-MY2"]}],"id":[{"id":"10.13039\/501100003711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Japanese encephalitis is a mosquito\u2010borne disease caused by Japanese encephalitis virus (JEV) infection. Although JEV infects and replicates in cells with multiple tissue origins, neurons are the preferential cells for JEV infection. Currently, the identities of JEV cell tropism are largely unclear. To gain better insight into the underlying identities of JEV cell tropism, this study was designed to compare the JEV cell tropism with na\u00efve or differentiated PC12 cells. Through nerve growth factor\u2010differentiated PC12 cells, we discovered that JEV efficiently replicated in differentiated PC12 cells rather than na\u00efve cells. Mechanistic studies revealed that viral adsorption\/attachment seemed not to be a crucial factor. Supporting data showed that antagonizing postreceptor intracellular signaling of interferons, along with the activation of suppressor of cytokine signaling\u20103 (SOCS3) expression and protein tyrosine phosphatase activity, were apparent in differentiated PC12 cells after JEV infection. Independent of differentiating inducing agents, the upregulation of SOCS3 expression and protein tyrosine phosphatase activity, as well as preferential JEV tropism, were common in JEV\u2010infected differentiated PC12 cells. Using cultured primary neurons, JEV efficiently replicated in embryonic neurons rather than adult neurons, and the preference was accompanied by higher SOCS3 expression and protein tyrosine phosphatase activity. Given that both SOCS3 and protein tyrosine phosphatases have been implicated in the process of neuronal differentiation, JEV infection seems to not only create an antagonizing strategy to escape host's interferon antiviral response but also takes advantage of cellular machinery to favor its replication. Taken together, current findings imply that dynamic changes within cellular regulators of antiviral machinery could be accompanied by events of neuronal differentiation, thus concurrently playing roles in the control of JEV cell tropism and replication. \u00a9 2017 IUBMB Life, 69(2):79\u201387, 2017<\/jats:p>","DOI":"10.1002\/iub.1595","type":"journal-article","created":{"date-parts":[[2017,1,23]],"date-time":"2017-01-23T09:58:49Z","timestamp":1485165529000},"page":"79-87","source":"Crossref","is-referenced-by-count":1,"title":["Susceptibility of na\u00efve and differentiated PC12 cells to Japanese encephalitis virus infection"],"prefix":"10.1002","volume":"69","author":[{"given":"Jian\u2010Ri","family":"Li","sequence":"first","affiliation":[{"name":"Division of Urology Taichung Veterans General Hospital Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih\u2010Cheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Taichung Veterans General Hospital Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Yi","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Surgery Feng Yuan Hospital Taichung City Taiwan"},{"name":"Graduate Institute of Pharmaceutical Science and Technology, Central Taiwan University of Science and Technology Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen\u2010Chuan","family":"Ou","sequence":"additional","affiliation":[{"name":"Division of Urology Taichung Veterans General Hospital Taichung City Taiwan"},{"name":"Department of Medical Research Taichung Veterans General Hospital Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih\u2010Yi","family":"Lin","sequence":"additional","affiliation":[{"name":"Center for Geriatrics and Gerontology, Taichung Veterans General Hospital Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Family Medicine Taichung Veterans General Hospital Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine National Chung Hsing University Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shue\u2010Ling","family":"Raung","sequence":"additional","affiliation":[{"name":"Department of Medical Research Taichung Veterans General Hospital Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Lan","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Medical Research Taichung Veterans General Hospital Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Jung","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Research Taichung Veterans General Hospital Taichung City Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology China Medical University Taichung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01233"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2010.01.008"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1128\/jvi.65.2.880-886.1991","article-title":"Japanese encephalitis virus neurotropism is dependent on the degree of neuronal maturity","volume":"65","author":"Ogata A.","year":"1991","journal-title":"J. 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