{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T08:14:54Z","timestamp":1777623294297,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T00:00:00Z","timestamp":1486684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science and Technology Planning Project of Guangdong Province, China","award":["2014A020212472"],"award-info":[{"award-number":["2014A020212472"]}]},{"name":"Science and Technology Planning Project of shenzhen, China","award":["JCYJ20150403101146288"],"award-info":[{"award-number":["JCYJ20150403101146288"]}]},{"name":"Health Bureau Health Scientific Research Projects of shenzhen, China","award":["201501003"],"award-info":[{"award-number":["201501003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aims to investigate the effects of TNF receptors associated factor 3 (TRAF3) on the signaling pathway and expression of downstream products of nuclear factor kappa B (NF\u2010\u03baB) in the epithelial cells of renal ducts in individuals with polycystic kidney disease (PKD). We observe the TRAF3 genic overexpression of the epithelial cells, which form a tubular branch structure, in polycystic kidneys and to explore the protective effect of TRAF3 on the cystogenesis and progression of PKD. Western blotting analysis was conducted to examine the signaling changes of NF\u2010\u03baB in PKD the epithelial cells and TRAF3 transgenic PKD epithelial cells. Changes in the downstream apoptosis factor and cell proliferation in PKD epithelial cells and TRAF3 transgenic PKD epithelial cells were detected. A three\u2010dimensional matrigel culture experiment was performed to examine abnormal tubulomorphogenesis <jats:italic>in vitro<\/jats:italic>. The overexpression of TRAF3 significantly inhibited the signaling pathway of NF\u2010\u03baB in the PKD epithelial cells, downregulated the expression of downstream factors Bcl\u20102 and Bcl\u2010xl, and significantly decreased cystic epithelial cell proliferation. Additional branch structures were observed in the PKD epithelial cells with a three\u2010dimensional culture compared to wildtype cells. TRAF3 may likely induce apoptosis and resistance to proliferation and may be a new target to inhibit the cyst formation in PKD by regulating the NF\u2010\u03baB signaling pathway Bcl\u20102 and Bcl\u2010xl activity. \u00a9 2017 IUBMB Life, 69(3):170\u2013178, 2017<\/jats:p>","DOI":"10.1002\/iub.1601","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T06:16:53Z","timestamp":1486707413000},"page":"170-178","source":"Crossref","is-referenced-by-count":7,"title":["TRAF3 delays cyst formation induced by NF\u2010\u03baB signaling"],"prefix":"10.1002","volume":"69","author":[{"given":"Liping","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Nephrology Shenzhen People's Hospital, Second Clinical Medical College, Jinan University Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaofeng","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, School of Medicine Jinan University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinzhou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Shenzhen People's Hospital, Second Clinical Medical College, Jinan University Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00406"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathol.2.010506.091850"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI72272"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1076"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2015.07.037"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.06.034"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg274"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.287656"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11831"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38716"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2011.01.025"},{"key":"e_1_2_6_13_1","first-page":"13251","article-title":"Constitutive activation of NF\u2010\u039aB causes tubule enlargement and cyst formation in cultured mammalian collecting duct cells","volume":"10","author":"Sun L. 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