{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T18:00:37Z","timestamp":1788199237928,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T00:00:00Z","timestamp":1486684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81171705"],"award-info":[{"award-number":["81171705"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Developmental dysplasia of the hip (DDH) is a developmental disorder that has long\u2010term chronic pain and limited hip joint mobility as major pathological characteristics. This study aims to access the association between the development of DDH and cartilage metabolic disorders. Cartilage tissue samples were acquired from patients with DDH, osteoarthritis (OA) and femoral neck fracture. The proteoglycan level was evaluated by safranin O\u2010fast green, toluidine blue and hematoxylin\u2010eosin (HE) staining. The levels of collagen\u2010II (Col\u2010II), collagen\u2010X (Col\u2010X) and metal matrix proteinase\u201013 (MMP\u201013) were evaluated by immunohistochemistry (IHC) and Western blotting analysis. The morphologic evaluation of cartilage was conducted by transmission electron microscopy (TEM). Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) was performed to detect the mRNA level of aggrecan, Col\u2010II, Col\u2010X and MMP\u201013. The aggrecan level in the cartilage matrix was significantly decreased in DDH patients by safranin O\u2010fast green and toluidine blue staining in comparison with that in the OA and control groups. In contrast with the OA group, the Col\u2010II expression was reduced while the MMP\u201013 expression increased in DDH patients, as shown by IHC and Western blotting analysis. The collagenous fibrils in cartilage of DDH patients appeared significantly sparse and disordered in the TEM analysis. In DDH patients, the mRNA expression levels of Col\u2010II and aggrecan were markedly reduced, while the mRNA expression of Col\u2010X was markedly increased, compared with the OA patients. There is severe articular cartilage degeneration in DDH patients. This observation provides us with new insight into cartilage metabolic regulation in DDH. \u00a9 2017 IUBMB Life, 69(3):179\u2013187, 2017<\/jats:p>","DOI":"10.1002\/iub.1606","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T06:16:43Z","timestamp":1486707403000},"page":"179-187","source":"Crossref","is-referenced-by-count":14,"title":["Severe cartilage degeneration in patients with developmental dysplasia of the hip"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8321-3655","authenticated-orcid":false,"given":"Wei\u2010Jia","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Xin Hua Hospital affiliated to Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Xin Hua Hospital affiliated to Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Xin Hua Hospital affiliated to Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010Feng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Xin Hua Hospital affiliated to Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Dong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Xin Hua Hospital affiliated to Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1302\/0301-620X.46B1.40","article-title":"Persistent joint laxity and congenital dislocation of the hip","volume":"46","author":"Carter C.","year":"1964","journal-title":"J. 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