{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:46:30Z","timestamp":1787240790345,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,11,11]],"date-time":"2017-11-11T00:00:00Z","timestamp":1510358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Science and Technology, Government of India"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Matrix metalloproteinases (\n                    <jats:italic>MMP<\/jats:italic>\n                    s) and tissue inhibitors of\n                    <jats:italic>MMP<\/jats:italic>\n                    s (\n                    <jats:italic>TIMP<\/jats:italic>\n                    s) are crucial to the processes of normal labor and parturition. We have previously reported aberrant protein levels of\n                    <jats:italic>MMP<\/jats:italic>\n                    s in placenta of women delivering preterm as compared to term. In this study, we examine the mRNA levels of\n                    <jats:italic>MMP<\/jats:italic>\n                    s (\n                    <jats:italic>MMP\u20101<\/jats:italic>\n                    ,\n                    <jats:italic>MMP\u20102<\/jats:italic>\n                    , and\n                    <jats:italic>MMP\u20109<\/jats:italic>\n                    ) and\n                    <jats:italic>TIMP<\/jats:italic>\n                    s (\n                    <jats:italic>TIMP\u20101<\/jats:italic>\n                    ,\n                    <jats:italic>TIMP\u20102<\/jats:italic>\n                    ) in the placenta from women delivering preterm as compared with term and further study the promoter DNA methylation of the\n                    <jats:italic>MMP\u20109<\/jats:italic>\n                    gene in a sub\u2010sample of term and preterm placentae. A total of 110 women were included in the study; 56 delivered term and 54 delivered preterm.\n                    <jats:italic>MMP<\/jats:italic>\n                    and\n                    <jats:italic>TIMP<\/jats:italic>\n                    mRNA levels were determined by Taqman\u2010based qPCR. Promoter CpG methylation of\n                    <jats:italic>MMP\u20109<\/jats:italic>\n                    gene was studied on a subset of 10 term and 8 preterm placenta using Epitect Methyl\u2010II PCR assay kit. The mRNA levels of\n                    <jats:italic>MMP\u20101<\/jats:italic>\n                    ,\u20102 were higher and those of\n                    <jats:italic>TIMP\u20101<\/jats:italic>\n                    ,\u20102 were lower in the placentae of women delivering preterm.\n                    <jats:italic>MMP\u20109<\/jats:italic>\n                    levels were comparable between the two groups. Among women undergoing spontaneous vaginal deliveries, higher mRNA levels of\n                    <jats:italic>MMP\u20101<\/jats:italic>\n                    ,\n                    <jats:italic>\u20102<\/jats:italic>\n                    and\n                    <jats:italic>\u20109<\/jats:italic>\n                    were seen in the placentae of those delivering preterm as compared to term. Similar results were seen in women undergoing preterm labor as compared to term.\n                    <jats:italic>MMP\u20109<\/jats:italic>\n                    gene promoter was hypomethylated in preterm placenta as compared to term placenta, while the mRNA levels were comparable between the two groups. The observed imbalance between\n                    <jats:italic>MMP<\/jats:italic>\n                    and\n                    <jats:italic>TIMP<\/jats:italic>\n                    expression may have prematurely triggered the signaling cascade leading to preterm birth. \u00a9 2017 IUBMB Life, 69(12):985\u2013993, 2017\n                  <\/jats:p>","DOI":"10.1002\/iub.1687","type":"journal-article","created":{"date-parts":[[2017,11,11]],"date-time":"2017-11-11T08:46:44Z","timestamp":1510390004000},"page":"985-993","source":"Crossref","is-referenced-by-count":33,"title":["Investigating the expression of\n                    <i>MMPs<\/i>\n                    and\n                    <i>TIMPs<\/i>\n                    in preterm placenta and role of CpG methylation in regulating\n                    <i>MMP\u20109<\/i>\n                    expression"],"prefix":"10.1002","volume":"69","author":[{"given":"Deepali","family":"Sundrani","sequence":"first","affiliation":[{"name":"Department of Nutritional Medicine Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Narang","sequence":"additional","affiliation":[{"name":"Department of Nutritional Medicine Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savita","family":"Mehendale","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Bharati Hospital and Research Center Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadhana","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Nutritional Medicine Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8514-3171","authenticated-orcid":false,"given":"Preeti","family":"Chavan\u2010Gautam","sequence":"additional","affiliation":[{"name":"Department of Nutritional Medicine Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.03.109"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004703-200512000-00008"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2393-10-S1-S2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719107304563"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2741\/2089"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000070112.80711.3D"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719110374115"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2007.04.001"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719107310308"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsgi.2004.04.001"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.87.3.8320"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0520"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029855"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.09.009"},{"key":"e_1_2_8_16_1","first-page":"1512","article-title":"Global signatures of protein and mRNA expression levels","volume":"5","author":"Sousa Abreu R.","year":"2009","journal-title":"Mol. 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