{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:25:46Z","timestamp":1771262746805,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,10,31]],"date-time":"2017-10-31T00:00:00Z","timestamp":1509408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"publisher","award":["PIP2015\u2010088"],"award-info":[{"award-number":["PIP2015\u2010088"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"publisher","award":["PUE 0089"],"award-info":[{"award-number":["PUE 0089"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"ANPCyT","doi-asserted-by":"publisher","award":["PICT 2015\u20100439"],"award-info":[{"award-number":["PICT 2015\u20100439"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lipopolysaccharides (LPS) are known to cause cholestasis in sepsis. There is evidence that a defective expression of canalicular aquaporin water channels contributes to bile secretory failure in LPS\u2010induced cholestasis. Thus, we studied whether the hepatic adenovirus\u2010mediated transfer of human aquaporin\u20101 gene (<jats:italic>haqp1<\/jats:italic>) can improve the cholestasis induced by LPS. Adenoviral vector encoding hAQP1 (AdhAQP1) or control vector was administered to rats by retrograde intrabiliary infusion. Hepatocyte canalicular hAQP1 expression was assessed by liver immunostaining and immunoblotting in purified plasma membranes. LPS reduced bile flow and biliary bile acid excretion by 30% and 45%, respectively. AdhAQP1\u2010treatment normalized both bile flow and biliary bile acid excretion in LPS\u2010induced cholestasis. Moreover, markedly elevated serum bile acid levels in cholestatic rats, were also normalized with the AdhAQP1 hepatic transduction. Bile flow and serum or biliary bile acids in normal rats were not significantly altered by AdhAQP1. AdhAQP1 delivery unaffected the downregulated protein expression of canalicular bile salt export pump (BSEP\/ABCB11) in cholestasis, but improved its transport activity restoring reduced canalicular cholesterol content. Our data suggest that the adenovirus\u2010mediated hepatocyte hAQP1 expression improves LPS\u2010induced cholestasis in rats by stimulating the BSEP\/ABCB11\u2010mediated biliary bile acid excretion; a finding that might contribute to the understanding and treatment of sepsis\u2010associated cholestatic diseases. \u00a9 2017 IUBMB Life, 69(12):978\u2013984, 2017<\/jats:p>","DOI":"10.1002\/iub.1689","type":"journal-article","created":{"date-parts":[[2017,10,31]],"date-time":"2017-10-31T10:28:46Z","timestamp":1509445726000},"page":"978-984","source":"Crossref","is-referenced-by-count":18,"title":["Adenovirus\u2010mediated human aquaporin\u20101 expression in hepatocytes improves lipopolysaccharide\u2010induced cholestasis"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2934-8863","authenticated-orcid":false,"given":"Julieta","family":"Marrone","sequence":"first","affiliation":[{"name":"Instituto de Fisiolog\u00eda Experimental, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario  Santa Fe Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauro","family":"Danielli","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda Experimental, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario  Santa Fe Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar I.","family":"Gaspari","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda Experimental, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario  Santa Fe Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal A.","family":"Marinelli","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda Experimental, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario  Santa Fe Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70238-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00232.2007"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90410.2008"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.7.3268"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-79885-9_20"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1210662109"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2014.78"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28564"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706148"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470110249.ch3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.276.1.G280"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00231.2003"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(00)70425-2"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v111.pm8698199"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-14541-4_5"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(03)00098-5"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2003.03053.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M808667200"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.108.024778"},{"key":"e_1_2_7_22_1","first-page":"6, 2642","article-title":"Hepatocyte aquaporins in bile formation and cholestasis","author":"Marinelli R. 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