{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:21:11Z","timestamp":1773296471723,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T00:00:00Z","timestamp":1516838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["BD\u20108937"],"award-info":[{"award-number":["BD\u20108937"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis E virus (HEV) infection remains a serious threat to life and productivity in developing world. Vaccine seems to be an effective, safe, and affordable approach to address HEV disease burden. The HEV genome consists of three open reading frames (ORFs). Of these, ORF2 encodes a single structural protein (pORF2) for the HEV capsid which has been studied extensively as vaccine candidates. Recently, it has been recognized that autophagy plays an important role in innate and adaptive immunity defense against intracellular pathogens. This mechanism could therefore promote a protective immune response by inducing CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells. In this study, HEV 239 and Beclin1 proteins were expressed in prokaryotic host cell [<jats:italic>Escherichia coli<\/jats:italic> (BL21)]. HEV 239 protein with different formulations (+Alum, +Beclin1, and +Alum\u2010Beclin1) were used as candidate vaccines and administrated subcutaneously in BALB\/c mice on 0, 14, and 28 days. Finally, elicited cellular and humoral immunity were evaluated. Taken together, although our results indicated that mice immunized with HEV 239 protein formulated with Alum, Beclin1, and Alum\u2009+\u2009Beclin1 displayed humoral and cellular response that was not significant in comparison with each other (<jats:italic>P &gt;<\/jats:italic> 0.05); whereas they were significant while compared with control groups (<jats:italic>P &lt;<\/jats:italic> 0.05). A comprehensive understanding of the intricate interplay between autophagy and immune response remains to be unraveled. Further study will clear the detailed impact of autophagy manipulation to enhance vaccine efficacy and boost the immune responses against the disease. \u00a9 2018 IUBMB Life, 70(3):207\u2013214, 2018<\/jats:p>","DOI":"10.1002\/iub.1719","type":"journal-article","created":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T16:56:47Z","timestamp":1516899407000},"page":"207-214","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of autophagy induction on <scp>HEV<\/scp> 239 vaccine immune response in a mouse model"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8752-5835","authenticated-orcid":false,"given":"Mehdi","family":"Khateri","sequence":"first","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asghar","family":"Abdoli","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Motevalli","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Fotouhi","sequence":"additional","affiliation":[{"name":"Department of Influenza and Other Respiratory Viruses Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azam","family":"Bolhassani","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arash","family":"Arashkia","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ehsan Ollah","family":"Jazaeri","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sepideh","family":"Shahbazi","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parvaneh","family":"Mehrbod","sequence":"additional","affiliation":[{"name":"Department of Influenza and Other Respiratory Viruses Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamed","family":"Naziri","sequence":"additional","affiliation":[{"name":"Department of Virology, School of Public Health Tehran University of Medical Science Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Reza","family":"Aghasadeghi","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph10104507"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2015.1008870"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-061516-044820"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000455"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/15216540701230503"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2010.191"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2016.12.011"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1928"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.12.018"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmq006"},{"key":"e_1_2_7_12_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-3-53"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.02.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.02.025"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-009-0335-7"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.05.064"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1720"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00167"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200105)31:5<1485::AID-IMMU1485>3.0.CO;2-A"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.11.075"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.8586-8596.1998"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.05.008"}],"container-title":["IUBMB Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fiub.1719","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iub.1719","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T04:07:57Z","timestamp":1695614877000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/iub.1719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,25]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1002\/iub.1719"],"URL":"https:\/\/doi.org\/10.1002\/iub.1719","archive":["Portico"],"relation":{},"ISSN":["1521-6543","1521-6551"],"issn-type":[{"value":"1521-6543","type":"print"},{"value":"1521-6551","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,25]]}}}