{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:16:57Z","timestamp":1790000217056,"version":"4.0.1"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T00:00:00Z","timestamp":1520553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prostate cancer (PCa) is considered the most common malignancy in men. The aim of this study is to assess the role of serum miR\u201015a and miR\u201016\u20101 expression in PCa development, diagnosis and prognosis aiming to find a specific noninvasive biomarker. This study comprised 70 patients with PCa, 70 patients complaining of benign prostatic hyperplasia (BPH), 30 patients with chronic prostatitis and 70 controls. Circulating miR\u201015a and miR\u201016\u20101 expression was detected by real\u2010time polymerase chain reaction. Prostate specific antigen levels were measured by enzyme\u2010linked immunosorbent assay. The expression levels of serum miR\u201015a were decreased in PCa patients compared with controls, chronic prostatitis and BPH patients (0.43\u2009\u00b1\u20090.12, 1.7\u2009\u00b1\u20090.76, 1.56\u2009\u00b1\u20090.34 and 1.53\u2009\u00b1\u20090.65, respectively). The expression levels of serum miR\u201016\u20101 were decreased in PCa patients compared with controls, chronic prostatitis and BPH patients (0.55\u2009\u00b1\u20090.23, 2.15\u2009\u00b1\u20090.87, 2.08\u2009\u00b1\u20090.54 and 1.96 \u00b10.61, respectively). Downregulation of miR\u201015a and miR\u201016\u20101 correlated with higher Gleason score (<jats:italic>P<\/jats:italic>\u2009=\u20090.002 and <jats:italic>P<\/jats:italic>\u2009=\u20090.006, respectively), higher tumor stage (<jats:italic>P<\/jats:italic>\u2009=\u20090.001 and <jats:italic>P<\/jats:italic>\u2009=\u20090.01, respectively), PCa metastasis (<jats:italic>P<\/jats:italic>\u2009=\u20090.002 and <jats:italic>P<\/jats:italic>\u2009=\u20090.025, respectively) and lymph node involvement (<jats:italic>P<\/jats:italic>\u2009=\u20090.02 and <jats:italic>P<\/jats:italic>\u2009=\u20090.007, respectively). Moreover, Receiver operating characteristic curve analysis revealed that combined miR\u201015a\/miR\u201016\u20101 and PSA increased the sensitivity and specificity for the diagnosis of PCa (97.1% and 94.3%, respectively) more than prostate specific antigen alone (82.9% sensitivity and 75.7% specificity). Combined serum miR\u201015a\/miR\u201016\u20101 expression and PSA level can be used as promising specific noninvasive biomarkers in the diagnosis and prognosis of PCa better than prostate specific antigen alone. \u00a9 2018 IUBMB Life, 70(5):437\u2013444, 2018<\/jats:p>","DOI":"10.1002\/iub.1733","type":"journal-article","created":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T16:12:44Z","timestamp":1520611964000},"page":"437-444","source":"Crossref","is-referenced-by-count":35,"title":["Diagnostic and prognostic value of serum miR\u201015a and miR\u201016\u20101 expression among egyptian patients with prostate cancer"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2292-9131","authenticated-orcid":false,"given":"Haidy E.","family":"Zidan","sequence":"first","affiliation":[{"name":"Medical Biochemistry and Molecular Biology Department, Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2241-1586","authenticated-orcid":false,"given":"Rehab S.","family":"Abdul\u2010Maksoud","sequence":"additional","affiliation":[{"name":"Medical Biochemistry and Molecular Biology Department, Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walid S. H.","family":"Elsayed","sequence":"additional","affiliation":[{"name":"Pathology Department, Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esam A. M.","family":"Desoky","sequence":"additional","affiliation":[{"name":"Urology Department, Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.5173\/ceju.2015.513","article-title":"Contemporary hormone therapy with LHRH agonists for prostate cancer: Avoiding osteoporosis and fracture","volume":"68","author":"Wilson H. C.","year":"2015","journal-title":"Cent. Eur. J. 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