{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:37:29Z","timestamp":1787499449721,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,14]],"date-time":"2018-03-14T00:00:00Z","timestamp":1520985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Biotechnology, India","award":["BT\/PR7173\/FNS\/20\/698\/2012"],"award-info":[{"award-number":["BT\/PR7173\/FNS\/20\/698\/2012"]}]},{"name":"Department of Biotechnology, India","award":["BT\/PR10658\/PFN\/20\/806\/2013"],"award-info":[{"award-number":["BT\/PR10658\/PFN\/20\/806\/2013"]}]},{"name":"University Grants Commission, Government of India, India"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Altered activity of the proteolytic machine\u2014the 26S proteasome is observed in many disease conditions. Hence, either inhibition or activation of the 26S proteasome is thought to be a novel therapy for treatment of certain diseases such as cancer and neurodegenerative disorders. In this study, we tested the potential of cinnamon and one of its active ingredients, procyanidin\u2010B2 (PCB2), in inhibiting the catalytic activities of the proteasome and suppressing prostate cancer cell growth. Proteasome activities were measured using fluorogenic substrates specific for the different enzymatic activities of the 26S proteasome by flourometry. Cell viability was assessed using the 3\u2010[4, 5\u2010dimethylthiazol\u20102\u2010yl]\u20102.5\u2010diphenyl\u2010tetrazolium bromide assay, while apoptosis was examined by Hoechst and propidium iodide staining and caspase\u20103 activity. Both, the cinnamon extract and its PCB2\u2010enriched F2 fraction inhibited the catalytic activities of the purified proteasome and the proteasome in cancer cells but not in normal cells. Furthermore, cinnamon and its active component decreased cell proliferation of human prostate cancer cells but not normal lung cells, decreased expression of anti\u2010apoptotic and angiogenic markers in prostate cancer cell lysates. These results demonstrate that cinnamon extract and its PCB2\u2010enriched fraction act as proteasome inhibitors and have prospects as anti\u2010cancer agents. \u00a9 2018 IUBMB Life, 70(5):445\u2013457, 2018<\/jats:p>","DOI":"10.1002\/iub.1735","type":"journal-article","created":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T08:16:03Z","timestamp":1521101763000},"page":"445-457","source":"Crossref","is-referenced-by-count":37,"title":["Procyanidin\u2010B2 enriched fraction of cinnamon acts as a proteasome inhibitor and anti\u2010proliferative agent in human prostate cancer cells"],"prefix":"10.1002","volume":"70","author":[{"given":"Srividya","family":"Gopalakrishnan","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism National Institute of Nutrition Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harshavardhana H.","family":"Ediga","sequence":"additional","affiliation":[{"name":"Department of Biochemistry National Institute of Nutrition Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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