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As reversible deacetylation of histone proteins modulate gene expression, we examined the effect of valproic acid (VPA) as selective histone deacetylase inhibitor on CCl\u20104 induced liver fibrosis. Thirty rats were divided into three equal groups; control group, fibrotic group and VPA\u2010treated group. The rats were sacrificed after 6 weeks of liver fibrosis induction. The histopathological effect on liver tissue was examined. The expression of \u03b1\u2010SMA and Smad\u20104 mRNA and serum levels of TGF\u2010\u03b21, alanine aminotransferase, and aspartate aminotransferase were determined. Treatment of rats with VPA attenuated carbon tetrachloride\u2010induced liver fibrosis. Moreover, \u03b1\u2010SMA and Smad\u20104 expression was repressed under VPA treatment and both serum TGF\u2010\u03b21 and liver enzymes were significantly decreased. The histone deacetylase inhibitor\u20101 VPA inhibits the epithelial\u2013mesenchymal transition and affects hepatic stellate cell activation during liver fibrosis through downregulation of Smad4 and \u03b1\u2010SMA expression which may serve as a promising agent in liver fibrosis treatment. \u00a9 2018 IUBMB Life, 70(6):511\u2013518, 2018<\/jats:p>","DOI":"10.1002\/iub.1742","type":"journal-article","created":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T13:55:18Z","timestamp":1522418118000},"page":"511-518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effect of histone deacetylase inhibitor on epithelial\u2010mesenchymal transition of liver fibrosis"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3396-2073","authenticated-orcid":false,"given":"Maggie M.","family":"Ramzy","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Minia University  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hend M","family":"Abdelghany","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Minia University  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nagwa M.","family":"Zenhom","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Minia University  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nashwa F.","family":"El\u2010Tahawy","sequence":"additional","affiliation":[{"name":"Department of Histology and Cell Biology, Faculty of Medicine Minia University  Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2015.06.016"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2015.296"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.02.004"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2013.02.019"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3758(PMID:"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.080172"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20070779"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.110.168385"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.02.006"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(02)00092-3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145985"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019052-200304000-00014"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nurt.2006.11.007"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2009.04.007"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005111187"},{"key":"e_1_2_5_17_1","first-page":"262","article-title":"Blocking of SMAD4 expression by shRNA effectively inhibits fibrogenesis of human hepatic stellate cells","volume":"8","author":"Khanizadeh S.","year":"2015","journal-title":"Gastroenterol. 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