{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T11:18:44Z","timestamp":1773227924476,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T00:00:00Z","timestamp":1536796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This study investigated the effect of muscle\u2010derived stem cells (MDSCs) and adipose tissue\u2010derived stem cells (ADSCs) in the treatment of stress urinary incontinence (SUI) and their differences in a rat model. MDSCs and ADSC were isolated from rats (<jats:italic>n<\/jats:italic> = 10), examined for their properties, and labeled with enhanced green fluorescent protein (EGFP) and \u03b2\u2010galactosidase (\u03b2\u2010gal) gene. Rats received bladder\u2010neck and transurethral sphincter injection of EGFP\u2010labeled MDSCs and \u03b2\u2010gal gene\u2010labeled ADSC and injection of D\u2010Hanks as a control (<jats:italic>n<\/jats:italic> = 24 each group). At 0, 15, 30, and 60 days after cells injection, urinary voiding function was assessed by urine dynamics detector. The rats were killed to harvest their urethras for tracking of MDSCs and ADSC. Western blotting and quantitative real\u2010time reverse transcription PCR (qRT\u2010PCR) was performed to detect smooth muscle contents. Urodynamic test showed that MDSCs and ADSC improved the function of urination in rats with intrinsic sphincter deficiency (ISD), and effect of MDSCs\u2010treatment was more pronounced. In addition, histologic analysis showed that the MDSCs and ADSC\u2010treated groups had significantly higher myosin and \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) content than the control group. Compared with ADSC\u2010treated groups, the MDSCs\u2010treated groups in myosin and \u03b1\u2010SMA content showed the tendency of increase. In summary, MDSCs and ADSCs have obvious effects in the treatment and\/or prevention of ISD and transplantation of MDSCs is more effective than ADSC. \u00a9 2018 IUBMB Life, 70(10):976\u2013984, 2018<\/jats:p>","DOI":"10.1002\/iub.1896","type":"journal-article","created":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T16:33:58Z","timestamp":1536856438000},"page":"976-984","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A Functional Comparison of Treatment of Intrinsic Sphincter Deficiency with Muscle\u2010Derived and Adipose Tissue\u2010Derived Stem Cells"],"prefix":"10.1002","volume":"70","author":[{"given":"Lingang","family":"Cui","sequence":"first","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6621-0017","authenticated-orcid":false,"given":"Qingjun","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zechen","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yudong","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengchao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianfei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haizhou","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengchao","family":"Lian","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000450759.34453.31"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2006.01426.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001920200023"},{"key":"e_1_2_6_5_1","first-page":"188","article-title":"Predicting intrinsic urethral sphincter dysfunction in women with stress urinary incontinence","volume":"84","author":"Horbach N. 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