{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:23:42Z","timestamp":1785907422939,"version":"3.56.0"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T00:00:00Z","timestamp":1534550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009530","name":"Jawaharlal Institute Of Postgraduate Medical Education and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IUBMB Life"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Interplay between the apoptosis, DNA damage, and oxidative stress as a host response to dengue viral infections remains unclear. Peripheral blood mononuclear cells (PBMCs) were isolated from 60 dengue infected patients, 20 patients with febrile illness other than dengue (OFI) and 10 non\u2010febrile illness (NFI) patients. DNA damage in the PBMCs was assessed using single cell gel electrophoresis and stages of apoptosis underwent by the PBMCs were studied by Annexin\u2013PI staining using flow cytometry. Plasma levels of malondialdehyde levels were estimated using thiobarbituric acid assay. Dengue infected individuals had showed increased DNA damage than NFI and OFI controls at the time of admission. Annexin\u2013PI staining revealed increased frequency of apoptotic cells in dengue infected PBMCs than controls during the admission time. Similar pattern was observed in samples collected around defervescence. Within the dengue cases, percentage of live cells was higher in non\u2010severe dengue than severe dengue at both the time points. Follow\u2010up samples in dengue showed less number of live cells and higher percentage of apoptotic cells with respect to their baseline and this was reversed in case of OFI. Plasma malondialdehyde levels were found to be relatively higher in dengue cases than controls at admission and around defervescence. Significant positive correlation between DNA damage, apoptosis, and plasma malondialdehyde levels might pave a way for understanding the complex interactions between virus and hosts response thereby aids in identifying plausible immunopathological links contributing to disease pathogenesis. \u00a9 2018 IUBMB Life, 70(11):1133\u20131143, 2018<\/jats:p>","DOI":"10.1002\/iub.1925","type":"journal-article","created":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T10:54:37Z","timestamp":1534589677000},"page":"1133-1143","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Lipid peroxidation, DNA damage, and apoptosis in dengue fever"],"prefix":"10.1002","volume":"70","author":[{"given":"Cleetus","family":"Cherupanakkal","sequence":"first","affiliation":[{"name":"Department of Biochemistry Jawaharlal Institute of Postgraduate Medicine and Research  Puducherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy Mariya","family":"Samadanam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Jawaharlal Institute of Postgraduate Medicine and Research  Puducherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishna Raja","family":"Muthuraman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Jawaharlal Institute of Postgraduate Medicine and Research  Puducherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surya","family":"Ramesh","sequence":"additional","affiliation":[{"name":"School of Chemical and Biotechnology SASTRA University  Thanjavur Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrit","family":"Venkatesan","sequence":"additional","affiliation":[{"name":"Central Inter\u2010Disciplinary Research Facility (CIDRF) A Unit of Sri Balaji Vidyapeeth (Deemed to\u2010be University) Educational and Charitable Public Trust  Puducherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6543-2977","authenticated-orcid":false,"given":"Agiesh Kumar","family":"Balakrishna Pillai","sequence":"additional","affiliation":[{"name":"Central Inter\u2010Disciplinary Research Facility (CIDRF) A Unit of Sri Balaji Vidyapeeth (Deemed to\u2010be University) Educational and Charitable Public Trust  Puducherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4908-2372","authenticated-orcid":false,"given":"Soundravally","family":"Rajendiran","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Jawaharlal Institute of Postgraduate Medicine and Research  Puducherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,18]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"WHO. 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